Recombinant Human alpha-L-Iduronidase/IDUA Avi Protein, CF

Name: Recombinant Human alpha-L-Iduronidase/IDUA Avi Protein, CF
Brand: R&D Systems
SKU: AVI11613

R&D Systems, part of Bio-Techne | Catalog # AVI11613

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Product Datasheet / COA / SDS

Fc Chimera

Key Product Details

Source:	CHO
Structure / Form:	Biotinylated via Avi-tag
Applications:	Bioactivity, Enzyme Activity

View Full Technical Details for Recombinant Human alpha-L-Iduronidase/IDUA Avi Protein, CF

Technical Data
Citations / Reviews

Product Specifications for Recombinant Human alpha-L-Iduronidase/IDUA Avi Protein, CF

Source

Chinese Hamster Ovary cell line, CHO-derived human alpha-L-Iduronidase/IDUA protein

Human IDUA (Ala26-Pro653) Accession # P35475.2	Human IgG₁ (Pro100-Lys330)	Avi-tag
N-terminus		C-terminus

Endotoxin level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal sequence Analysis

Ala26

Predicted Molecular Mass

98 kDa, under reducing conditions

SDS-PAGE

98 kDa, under reducing conditions

Activity

Measured by its binding ability in a functional ELISA. Recombinant Human alpha‑L‑Iduronidase/IDUA Fc Chimera Avi-tag binds to Human alpha -L-Iduronidase/IDUA Antibody (Catalog # AF4119) with an ED₅₀ of 2.00-20.0 ng/mL. Measured by its ability to cleave a fluorogenic substrate, 4-Methylumbelliferyl alpha -L-iduronide. The specific activity is >4500 pmol/min/μg, as measured under the described conditions.

Scientific Data Examples for Recombinant Human alpha-L-Iduronidase/IDUA Avi Protein, CF

	Biotinylated Recombinant Human alpha‑L‑Iduronidase/ IDUA Fc Chimera Avi-tag Protein Binding Activity. Measured by its binding ability in a functional ELISA. Recombinant Human alpha-L-Iduronidase/IDUA Fc Chimera Avi-tag Protein (Catalog # AVI11613) binds to Human alpha -L-Iduronidase/ IDUA Antibody (AF4119) with an ED₅₀ of 2.00‑20.0 ng/mL.
	Biotinylated Recombinant Human alpha‑L‑Iduronidase/IDUA Fc Chimera Avi-tag Protein SDS-PAGE. 2 μg/lane of Biotinylated Recombinant Human alpha‑L‑Iduronidase/IDUA Fc Chimera Avi-tag Protein (Catalog # AVI11613) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 104-115 kDa and 210-230 kDa, respectively.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

AVI11613

Formulation	Supplied as a 0.2 μm filtered solution in Sodium Acetate, NaCl and Glycerol.
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20 to -70 °C as supplied. 3 months, -20 to -70 °C under sterile conditions after opening.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: alpha-L-Iduronidase/IDUA

a-L-Iduronidase is a member of the glycoside hydrolase family encoded by the IDUA gene (1). It is an important enzyme required for the lysosomal degradation of glycosaminoglycans (GAGS) and hydrolyzes the non-reducing terminal a-L-iduronic acid residues in GAGS including dermatan sulfate and heparan sulfate. Human IDUA is a 653 aa protein composed of a signal peptide removed in the lysosome for mature form and three domains: a triosephosphate isomerase barrel fold containing the catalytic site, a B-sandwich domain, and an Ig(Ig)-like domain. The protein has six reported N-glycosylation sites and the glycosylation status of the enzyme correlates with its catalytic activity (1). More than a hundred disease-associated variants in the IDUA gene have been identified (1, 2). Mutations in IDUA that result in enzymatic deficiency lead to the autosomal recessive disease mucopolysaccharidosis type I (MPS I) (3). MPS I can be classified into three clinical subtypes; Hurler syndrome, Hurler-Scheie syndrome, and Scheie syndrome with decreasing severity, respectively. MPS I causes progressive cellular, tissue and organ damage, and several clinical studies using enzyme replacement therapy show positive results (4,5). More recently, the IDUA gene has been linked to osteoporosis (6, 7).

Item	Value
References	Maita, N. et al. (2013) Proc. Natl. Acad. Sci. 110:14628. Borges, P. et al. (2021) Front. Mol. Biosci. 8:752797. Scott, H.S. et al. (1995) Hum. Mutat. 6:288. Wraith, J.E. (2005) Expert Opin. Pharmacother. 6:489. Jameson, E. (2016) Cochrane Database Syst. Rev. 4:CD009354. Kodric, K. et al. (2016) Wien Klin Wochenschr. 128:480. Niu, T. et al. (2016) J. Bone Miner. Res. 31:358.
Entrez Gene IDs	3425 (Human); 15932 (Mouse); 360904 (Rat); 102116889 (Cynomolgus Monkey)
Alternate Names	IDA, IDUA, MPS1, MPSI, alpha-L-Iduronidase, alphaLIduronidase

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Recombinant Human alpha-L-Iduronidase/IDUA Avi Protein, CF

R&D Systems, part of Bio-Techne | Catalog # AVI11613

Key Product Details

Product Specifications for Recombinant Human alpha-L-Iduronidase/IDUA Avi Protein, CF

Scientific Data Examples for Recombinant Human alpha-L-Iduronidase/IDUA Avi Protein, CF

Formulation, Preparation and Storage

Carrier Free

Reconstitution Calculator

Background: alpha-L-Iduronidase/IDUA

Customer Reviews for Recombinant Human alpha-L-Iduronidase/IDUA Avi Protein, CF

Product Documents for Recombinant Human alpha-L-Iduronidase/IDUA Avi Protein, CF

Product Datasheets

COA

Certificate of Analysis

SDS

CONTACT INFORMATION

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