Recombinant Human AMCase/CHIA Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 9859-GH

R&D Systems, part of Bio-Techne
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9859-GH-050
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Key Product Details

Source

HEK293

Accession #

Q9BZP6-1

Conjugate

Unconjugated

Applications

Enzyme Activity

View all AMCase/CHIA Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human AMCase/CHIA protein
Tyr22-Pro408, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Tyr22

Predicted Molecular Mass

44 kDa

SDS-PAGE

39-47 kDa, reducing conditions

Activity

Measured by its ability to cleave 4-Methylumbelliferyl-beta -D-N,N’,N”-triacetylchitotriose (4-MU-TACT).
The specific activity is >125 pmol/min/μg, as measured under the described conditions.

Formulation, Preparation and Storage

9859-GH
Formulation Supplied as a 0.2 μm filtered solution in Tris and NaCl.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: AMCase/CHIA

Human acidic mammalian chitinase (AMCase), or CHIA, degrades chitin and chitotriose, therefore participates in the defense against nematodes, fungi and other pathogens (1, 2, 3). It is one of the important proteins involved in T-helper cell type 2 (Th2)-mediated inflammation and has been implicated in asthma and allergic diseases (4). It protects lung epithelial cells against apoptosis possibly via promoting phosphorylation of AKT1 and stimulating chemokine production (5, 6). Inhibition of AMCase results in decreased airway inflammation and airway hyper-responsiveness in a mouse asthma model. Its function in the inflammatory response and in protecting cells against apoptosis is inhibited by allosamidin, suggesting that the function of this protein depends on carbohydrate binding (7).

References

  1. Boot, R.G. et al. (2001) J. Biol. Chem. 276:6770.
  2. Seibold, M.A. et al. (2009) J. Biol. Chem. 284:19650.
  3. Chou, Y.T. et al. (2006) Biochemistry 45:4444.
  4. Zhu, Z, et al. (2004) Science 304:1678.
  5. Hartl, D. et al. (2008) J. Biol. Chem. 283:33472.
  6. Hartl, D. et al. (2009) J. Immunol. 182:5098.
  7. Ober, C. and Chupp, G.L. (2009) Curr. Opin. Allergy Clin. Immunol. 9:401.

Long Name

Acidic Mammalian Chitinase

Alternate Names

CHIA, CHIT2, Chitinase, Acidic, Lung-Specific Protein TSA1902

Entrez Gene IDs

27159 (Human); 81600 (Mouse); 113901 (Rat)

Gene Symbol

CHIA

UniProt

Q9BZP6-1

Additional AMCase/CHIA Products

Product Documents for Recombinant Human AMCase/CHIA Protein, CF

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Product Specific Notices for Recombinant Human AMCase/CHIA Protein, CF

For research use only

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