Recombinant Human AMIGO3 Fc Chimera Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 9508-AM

R&D Systems, part of Bio-Techne
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9508-AM-050
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Key Product Details

Source

CHO

Accession #

Q86WK7

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all AMIGO3 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human AMIGO3 protein
Human AMIGO3
(Thr20-Pro378)
Accession # Q86WK7		 IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Thr20

Predicted Molecular Mass

66 kDa

SDS-PAGE

83-103 kDa, reducing conditions

Activity

Measured by its ability to inhibit IL-2 secretion by mouse T cells in the presence of anti-CD3.
The ED50 for this effect is 1-5 μg/mL.

Scientific Data Images for Recombinant Human AMIGO3 Fc Chimera Protein, CF

Recombinant Human AMIGO3 Fc Chimera Protein Bioactivity

Recombinant Human AMIGO3 Fc Chimera Protein Bioactivity

Recombinant Human AMIGO3 Fc Chimera (Catalog # 9508‑AM) inhibits IL-2 secretion by mouse T cells in the presence of anti-CD3 antibody. The ED50 for this effect is 1-5 μg/mL.

Formulation, Preparation and Storage

9508-AM
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 200 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
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Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: AMIGO3

AMIGO3 (Amphoterin-induced protein 3), also called Alivin-3, is a 68 kDa single-pass type I membrane cell adhesion protein. AMIGO3 belongs to a family of leucine-rich repeat (LRR) containing proteins that play various roles in nervous system development and function (1, 2). Human AMIGO3 cDNA encodes 504 amino acids (aa) including a 19 aa signal sequence, 264 aa extracelluar domain, 21 aa transmembrane domain and 100 aa cytoplasmic domain. Human AMIGO3 shares 77.2% and 79.1% aa sequence identity with mouse and rat AMIGO3, respectively. AMIGO family proteins are cell adhesion molecules that exhibit homophilic and heterophilic binding properties and are thought to play roles in neuronal axon tract development and cell adhesion. AMIGO3 is expressed in brain tissues, also found in liver, kidney and spleen (3). AMIGO3 mRNA and protein levels are preferentially and significantly raised in DRGN and RGC immediately after central axotomy. Depression of AMIGO3 expression correlates with dorsal column (DC) and optic nerve regeneration. AMIGO3 interacts with NgR1 and p75/TROY forming a functional receptor complex that activates RhoGTP in cells exposed to CNS myelin extracts (CME). AMIGO3 substitutes for LINGO-1 in centrally axotomized DRGN and RGC in the acute phase of injury. AMIGO3-NgR1-p75/TROY receptor complex mediates immediate axon growth inhibitory responses to CNS myelin (4). Our in-house data showed that AMIGO3 inhibited anti-CD3 induced IL-2 secretion on CD3+ cells, suggesting that AMIGO3 may be involved in T cell activation.

References

  1. Chen, Y. et al. (2006) Brain Res. Rev. 51:265.
  2. SwissProt Accession # Q86WK7.
  3. Kuja-Panula J, et al. (2003) J Cell Biol 160:963.
  4. Ahmed Z, et al. (2013) PLoS One. 8:e61878.

Long Name

Amphoterin-induced Gene and ORF 3

Alternate Names

adhesion molecule with Ig-like domain 3, ALI3, alivin-3, AMIGO-3, amphoterin-induced gene and ORF 3, amphoterin-induced protein 3, KIAA1851, MGC120552

Entrez Gene IDs

386724 (Human); 320844 (Mouse)

Gene Symbol

AMIGO3

UniProt

Q86WK7

Additional AMIGO3 Products

Product Documents for Recombinant Human AMIGO3 Fc Chimera Protein, CF

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Product Specific Notices for Recombinant Human AMIGO3 Fc Chimera Protein, CF

For research use only

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