Recombinant Human Aminopeptidase N/CD13 Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 3815-ZN
Key Product Details
Product Specifications
Source
Mouse myeloma cell line, NS0-derived human Aminopeptidase N/CD13 protein
Lys69-Lys967, with a C-terminal 10-His tag
Lys69-Lys967, with a C-terminal 10-His tag
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Lys69
Predicted Molecular Mass
104 kDa
SDS-PAGE
131 kDa, reducing conditions
Activity
Measured by its ability to cleave the fluorogenic peptide substrate, Ala-7-amido-4-methylcoumarin (Ala-AMC).
The specific activity is >2,500 pmol/min/µg, as measured under the described conditions.
The specific activity is >2,500 pmol/min/µg, as measured under the described conditions.
Scientific Data Images for Recombinant Human Aminopeptidase N/CD13 Protein, CF
Recombinant Human Aminopeptidase N/CD13 Protein Enzyme Activity
Recombinant Human Aminopeptidase N (Catalog # 3815-ZN) is measured by its ability to cleave the fluorogenic peptide substrate, Ala-7-amido-4-methylcoumarin (Ala-AMC).Formulation, Preparation and Storage
3815-ZN
| Formulation | Supplied as a 0.2 μm filtered solution in MES and NaCl. |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: Aminopeptidase N/CD13
The human ANPEP gene encodes aminopeptidase N (APN), which is also known as microsomal aminopeptiase, alanyl aminopeptidase, aminopeptidase M, CD13, or membrane protein p161 (1‑3). The deduced amino acid sequence of human APN consists of a short cytoplasmic tail (residues 2 to 8), a transmembrane region (residue 9 to 32), a Ser/Thr rich region and a zinc metalloprotease domain (residues 69 to 966). Widely expressed in many cells, tissues and species, APN cleaves the N-terminal amino acids from bioactive peptides, leading to their inactivation or degradation. The roles of APN in many fields, such as neuroscience, hematopoeitic cells, immune system, angiogenesis, cancer and viral infection, have been reviewed (3).
References
- Olsen, J. et al. (1988) FEBS Lett. 238:307.
- Look, A.T. et al. (1989) J. Clin. Invest. 83:1299.
- Turner, A.J. (2004) in Handbook of Proteolytic Enzymes (ed. Barrett, et al.) pp. 289, Academic Press, San Diego.
Alternate Names
Aminopeptidase M, ANPEP, APN, CD13, gp150, PEPN
Gene Symbol
ANPEP
UniProt
Additional Aminopeptidase N/CD13 Products
Product Documents for Recombinant Human Aminopeptidase N/CD13 Protein, CF
Product Specific Notices for Recombinant Human Aminopeptidase N/CD13 Protein, CF
For research use only
Loading...
Loading...
Loading...