Recombinant Human Angiopoietin-like 5 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 6675-AN/CF

R&D Systems, part of Bio-Techne
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6675-AN-025/CF
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Key Product Details

Source

CHO

Accession #

AAH49170

Conjugate

Unconjugated

Applications

Bioactivity

View all Angiopoietin-like Protein 5/ANGPTL5 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human Angiopoietin-like Protein 5/ANGPTL5 protein
Met1-Lys388 with a C-terminal 6-His tag

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Val23 & Asn26

Predicted Molecular Mass

42.3 kDa

SDS-PAGE

50-60 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human Angiopoietin-like Protein 5/ANGPTL5 (Catalog # 6675-AN/CF) is immobilized at 2 µg/mL (100 µL/well), Recombinant Human LILRB2/CD85d/ILT4 Fc Chimera (Catalog # 2078-T4) binds with an ED50 of 80.0-800 ng/mL.

Scientific Data Images for Recombinant Human Angiopoietin-like 5 Protein, CF

Recombinant Human Angiopoietin‑like Protein 5/ANGPTL5 Binding Activity.

When Recombinant Human Angiopoietin-like Protein 5/ANGPTL5 (Catalog # 6675-AN/CF) is immobilized at 2 µg/mL (100 µL/well), Recombinant Human LILRB2/CD85d/ILT4 Fc Chimera (2078-T4) binds with an ED50 of 80.0-800 ng/mL.

Formulation, Preparation and Storage

6675-AN/CF
Formulation Supplied as a 0.2 μm filtered solution in PBS and NaCl.
Reconstitution
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after opening.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: Angiopoietin-like Protein 5/ANGPTL5

Angiopoietin-like 5 (ANGPTL5) is a secreted glycoprotein that is structurally related to the angiopoietins which contain an N-terminal coiled‑coil domain and a C-terminal fibrinogen-like domain (1-3). It shares 88% and 93% aa sequence identity with bovine and porcine ANGPTL5, and greater than 98% identity with chimpanzee, orangutan, and rhesus ANGPTL5. ANGPTL5 is expressed in adipose, bronchial, epididymal, and heart tissue (3, 4). Rare polymorphisms and loss of function mutations in human ANGPTL5 are associated with low circulating triglyceride levels and alterations in body mass index (4, 5). ANGPTL5, when used in combination with other growth factors such as SCF, Thrombopoietin, IGF-II, FGF acidic, Flt-3 Ligand, and IGFBP-2, enhances the expansion and engraftment of human and mouse hematopoietic stem cells (6-8). The coiled-coil domain of ANGPTL proteins is critical for this effect. However, the molecular mechanism of ANGPTL proteins in stem cell activity remains unclear (6).

References

  1. Oike, Y. et al. (2009) Circ. J. 73:2192.
  2. Miida, T. and S. Hirayama (2010) Curr. Opin. Lipidol. 21:70.
  3. Zeng, L. et al. (2003) J. Hum. Genet. 48:159.
  4. Romeo, S. et al. (2009) J. Clin. Invest. 119:70.
  5. Liu, D.J. and S.M. Leal (2010) PloS Genet. 6:e1001156.
  6. Zhang, C.C. et al. (2006) Nat. Med. 12:240.
  7. Zhang, C.C. et al. (2008) Blood 111:3415.
  8. Khoury, M. et al. (2010) Stem Cells Dev. Dec 8 epub.

Alternate Names

Angiopoietin like Protein 5, ANGPTL5

Entrez Gene IDs

253935 (Human)

Gene Symbol

ANGPTL5

UniProt

AAH49170

Additional Angiopoietin-like Protein 5/ANGPTL5 Products

Product Documents for Recombinant Human Angiopoietin-like 5 Protein, CF

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Human Angiopoietin-like 5 Protein, CF

For research use only

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