Recombinant Human Angiopoietin-like Protein 2/ANGPTL2, CF

R&D Systems, part of Bio-Techne | Catalog # 9795-AN

C-Terminal Fragment
R&D Systems, part of Bio-Techne
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9795-AN-050
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Key Product Details

Source

CHO

Accession #

Q9UKU9.1

Conjugate

Unconjugated

Applications

Bioactivity

View all Angiopoietin-like Protein 2/ANGPTL2 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human Angiopoietin-like Protein 2/ANGPTL2 protein
Thr260-His493, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Thr260

Predicted Molecular Mass

28 kDa

SDS-PAGE

28-36 kDa, under reducing conditions.

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human Angiopoietin‑like Protein 2/ANGPTL2 is immobilized at 1 μg/mL, 100 μL/well, the concentration of Recombinant Human LILRB2/CD85d/ILT4 Fc Chimera Recombinant Human ILT4/CD85d Fc Chimera (Catalog # 2078-T4) that produces 50% of the optimal binding response is 6-48 ng/mL.

Reviewed Applications

Read 1 review rated 5 using 9795-AN in the following applications:

Scientific Data Images for Recombinant Human Angiopoietin-like Protein 2/ANGPTL2, CF

Recombinant Human Angiopoietin-like Protein 2/ANGPTL2 Binding Activity

Recombinant Human Angiopoietin-like Protein 2/ANGPTL2 Binding Activity

When Recombinant Human Angiopoietin-like Protein 2/ANGPTL2 (Catalog # 9795-AN) is immobilized at 1 µg/mL, 100 µL/well, Recombinant Human ILT4/CD85d Fc Chimera (Catalog # 2078-T4) binds with an ED50 of 6-48 ng/mL.

Formulation, Preparation and Storage

9795-AN
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 500 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
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Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Angiopoietin-like Protein 2/ANGPTL2

Angiopoietin-like Protein 2 (ANGPTL2), also known as ARP2 is a secreted 56 kDa glycoprotein that contains a N-terminal coiled coil domain and a C-terminal fibrinogen like domain (1). ANGPTL2 is one of the seven members of the Angiopoietin Like family proteins, which are structurally similar to angiopoietins (2). Within mature human ANGPTL2, amino acid 260-493 contains the C-terminal fibrinogen like domain, and this domain shares 99% sequence identity with both mouse and rat homologs. ANGPTL2 is widely expressed in many tissues, and its expression is induced by chronic, but not acute hypoxia (3). Elevated level of ANGPTL2 in the serum is also correlated with inflammation and obesity (3). ANGPTL2 has both pro and anti-angiogenic functions (1, 4). It serves as tumor suppressor in ovarian cancer (5), and also promote metastasis in various cancer types, making it potential biomarker for tumor progression (6-7). ANGPTL2 also function as growth factor to enhance the survival of hematopoietic progenitors, mediated by its coiled coil domain (8). Receptor of ANGPTL2 is recently identified as LILRB2, and both the coiled coil domain and fibrinogen like domain are required for optimal binding (9).

References

  1. Kim, I. et al. (1999) J Biol Chem 274:26523.
  2. Santulli, G. et al. (2014) Front Endocrinol 5:4.
  3. Tabata, M. et al. (2009) Cell Metab 10:178.
  4. Kubota, Y. et al. (2005) Proc. Natl. Acad. Sci. U.S.A. 102:13502.
  5. Kikuchi, R. et al. (2008) Cancer Res 68:5067.
  6. Odagiri, H. et al. (2014) Sci Signal 7:ra7.
  7. Endo, M. et al. (2012) Cancer Res 72:1784.
  8. Broxmeyer, H.E. et al. (2012) Blood Cells Mol Dis 48:25.
  9. Deng, M. et al. (2014) blood 124:924.

Alternate Names

ANGPTL2, ANGRP2

Entrez Gene IDs

23452 (Human); 26360 (Mouse)

Gene Symbol

ANGPTL2

UniProt

Q9UKU9.1

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Product Documents for Recombinant Human Angiopoietin-like Protein 2/ANGPTL2, CF

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Product Specific Notices for Recombinant Human Angiopoietin-like Protein 2/ANGPTL2, CF

For research use only

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