Recombinant Human Annexin A1 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 3770-AN

R&D Systems, part of Bio-Techne
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3770-AN-050
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Key Product Details

Source

E. coli

Accession #

P04083

Structure / Form

Monomer

Conjugate

Unconjugated

Applications

Bioactivity

View all Annexin A1 Proteins and Enzymes »

Product Specifications

Source

E. coli-derived human Annexin A1 protein
Met1-Asn346, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ala2

Predicted Molecular Mass

39.4 kDa

SDS-PAGE

38 kDa, reducing conditions

Activity

Measured by its ability to inhibit proliferation of A549 human lung carcinoma cells.
The ED50 for this effect is 1.0-4.0 μg/mL.

Formulation, Preparation and Storage

3770-AN
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 400 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Annexin A1

The Annexins comprise a family of proteins that are involved in many aspects of cellular membrane dynamics and the regulation of membrane‑associated proteins. They are characterized by multiple repeats of 60‑70 amino acids (aa) which assemble into a curved disc‑like domain that binds to membrane phospholipids in a calcium‑dependent manner (1). Human Annexin A1 (ANXA1), also known as Lipocortin 1, Calpactin II, Lipomodulin, and Chromobindin 9, contains four annexin repeats and shares approximately 88% aa sequence identity with mouse and rat Annexin A1 (2). Annexin A1 is found in the cytoplasm, on the cell surface, and in the extracellular fluid (3). The full length 37 kDa protein is susceptible to intracellular and extracellular proteolytic cleavages which generate various N‑terminal peptides and a truncated C‑terminal portion (3‑6). In addition to its membrane activities, Annexin A1 is a major effector of glucocorticoid‑induced immune suppression and growth arrest (7, 8). It associates with S100A11, and this complex inhibits Phospholipase A2 and the production of inflammatory eicosanoids (2, 4). Annexin A1 is released by apoptotic cells and also during neutrophil adhesion to vascular endothelial cells (3, 9). It binds the FPRL1/ALX receptor on neutrophils, while the cleaved N‑terminal aa 2‑26 peptide additionally binds FPR (10, 11). These interactions inhibit leukocyte activation and extravasation to sites of inflammation (3, 9, 10, 12). In contrast, the 33 kDa molecule (generated by the removal of aa 2‑26) promotes activation of vascular endothelial cells and neutrophil transendothelial migration (6). Annexin A1 phosphorylation at multiple locations is important for the regulation of its cleavage, membrane interactions, and ability to suppress ACTH production in the pituitary (4, 13, 14). Annexin A1 additionally regulates adaptive immune responses by enhancing T cell activation and promoting a Th1 bias (15). It can exert both positive and negative effects on epithelial‑mesenchymal transition and tumor metastasis (16‑18).

References

  1. Gerke, V. et al. (2005) Nat. Rev. Mol. Cell Biol. 6:449.
  2. Wallner, B.P. et al. (1986) Nature 320:77.
  3. Perretti, M. et al. (1996) Nat. Med. 2:1259.
  4. Sakaguchi, M. et al. (2007) J. Biol. Chem. 282:35679.
  5. Vong, L. et al. (2007) J. Biol. Chem. 282:29998.
  6. Williams, S.L. et al. (2010) J. Immunol. 185:3057.
  7. Perretti, M. and F. D’Acquisto (2009) Nat. Rev. Immunol. 9:62.
  8. Croxtall, J.D. et al. (1992) Proc. Natl. Acad. Sci. 89:3571.
  9. Pupjalis, D. et al. (2011) EMBO Mol. Med. 3:102.
  10. Perretti, M. et al. (2002) Nat. Med. 8:1296.
  11. Hayhoe, R.P. et al. (2006) Blood 107:2123.
  12. Gastardelo, T.S. et al. (2009) Am. J. Pathol. 174:177.
  13. Dorovkov, M.V. et al. (2011) Biochemistry 50:2187.
  14. McArthur, S. et al. (2009) FASEB J. 23:4000.
  15. D’Acquisto, F. et al. (2007) Blood 109:1095.
  16. Yi, M. and J.E. Schnitzer (2009) Proc. Natl. Acad. Sci. 106:17886.
  17. Maschler, S. et al. (2010) EMBO Mol. Med. 2:401.
  18. de Graauw, M. et al. (2010) Proc. Natl. Acad. Sci. 107:6340.

Alternate Names

ANX1, ANXA1, LPC1

Entrez Gene IDs

301 (Human); 16952 (Mouse); 25380 (Rat)

Gene Symbol

ANXA1

UniProt

P04083

Additional Annexin A1 Products

Product Documents for Recombinant Human Annexin A1 Protein, CF

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Product Specific Notices for Recombinant Human Annexin A1 Protein, CF

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