Recombinant Human Annexin A3 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 9410-AN

R&D Systems, part of Bio-Techne
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9410-AN-050
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Key Product Details

Source

E. coli

Accession #

P12429

Structure / Form


Conjugate

Unconjugated

View all Annexin A3 Proteins and Enzymes »

Product Specifications

Source

E. coli-derived human Annexin A3 protein
Ala2-Asp323, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ala2

Predicted Molecular Mass

37 kDa

SDS-PAGE

36 kDa, reducing conditions

Activity

Bioassay data are not available.

Formulation, Preparation and Storage

9410-AN
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 250 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
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Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Annexin A3

Annexin A3 (ANXA3), also known as Annexin III and Lipocortin III (LPC3), is a 37 kDa member of the Annexin family. Annexins are calcium-dependent phospholipid-binding proteins that are preferentially located on the cytosolic face of the plasma membrane. The Annexins consist of a unique N-terminal domain followed by a homologous C-terminal core domain containing the phospholipid-binding sites. The C-terminal domain is comprised of four 60‑70 aa annexin repeats which form a tightly packed disc known as the annexin domain. Members of the Annexin family play a role in cytoskeletal interactions, phospholipase inhibition, regulation of cellular growth, and intracellular signal transduction pathways (1). Annexin A3 is up-regulated in microglia following stroke and in parenchymal hepatocytes in regenerating liver (2, 3). It can be up- or down‑regulated in various carcinomas (4). Alternate splicing generates an additional isoform with a 30 amino acid (aa) deletion near the N-terminus (5). Human Annexin A3 shares 89% and 85% aa sequence identity with mouse and rat Annexin A3, respectively.

References

  1. Gerke, V. et al. (2005) Nat. Rev. Mol. Cell Biol. 6:449.
  2. Kessler, C., et al. (2007). Rom. J. Morphol. Embryol. 49:27.
  3. Harashima, M. et al. (2008). J. Biochem. 143:537.
  4. Wu, N., et al. (2013). Clin. Transl. Oncol. 15:106.
  5. Bianchi, C. et al. (2010). Am. J. Pathol. 176:1660.

Alternate Names

ANXA3, Calcimedin 35-alpha, Lipocortin III, PAP-III

Entrez Gene IDs

306 (Human); 11745 (Mouse); 25291 (Rat)

Gene Symbol

ANXA3

UniProt

P12429

Additional Annexin A3 Products

Product Documents for Recombinant Human Annexin A3 Protein, CF

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Product Specific Notices for Recombinant Human Annexin A3 Protein, CF

For research use only

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