Recombinant Human Arginase 1/ARG1 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 5868-AR

R&D Systems, part of Bio-Techne
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5868-AR-010
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Key Product Details

Source

E. coli

Accession #

P05089

Structure / Form

Monomer

Conjugate

Unconjugated

Applications

Enzyme Activity

View all Arginase 1/ARG1 Proteins and Enzymes »

Product Specifications

Source

E. coli-derived human Arginase 1/ARG1 protein
Met1-Lys322 with an N-terminal Met and 6-His tag

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Met

Predicted Molecular Mass

36 kDa

SDS-PAGE

40 kDa, reducing conditions

Activity

Measured by the production of urea during the hydrolysis of arginine.
The specific activity is >35,000 pmol/min/μg, as measured under the described conditions.

Formulation, Preparation and Storage

5868-AR
Formulation Supplied as a 0.2 μm filtered solution in Tris, NaCl, Glycerol and DTT.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.

Background: Arginase 1/ARG1

Arginase 1, also known as liver arginase, is a binuclear manganese metalloenzyme. It is a key enzyme of the urea cycle that catalyses the conversion of L-arginine into L-ornithine and urea, the final cytosolic reaction of urea formation in the mammalian liver (1). Arginase 1 is abundantly expressed in liver, but it is also expressed in cells and tissues that lack a complete urea cycle, including lung. Arginase is a critical regulator of nitric oxide synthesis and vascular function (2). It is implicated in a variety of human diseases including vascular disease, pulmonary disease, infectious disease, immune cell function and cancer (3). In humans, hereditary defects in arginase result in an accumulation of arginine in the blood known as hyperarginemia (4). Arginase deficiency can also result in the accumulation of nitrogen in the form of ammonia, which results in hyperammonemia (5).

References

  1. Dowling, D. et al. (2008) Cell Mol. Life Sci. 65:2039.
  2. Durante, W. et al. (2007) Clin. Exp. Pharmacol. Physiol. 34:906.
  3. Morris, S. (2009) Br. J. Pharmacol. 157:922.
  4. Crombez, E. et al. (2005) Mol. Genet. Metab. 84:243.
  5. Scaglia, F. et al. (2004) J. Nutr. 134:2775s.

Long Name

Liver-Type Arginase

Alternate Names

AI, ARG1, Arginase-1, Liver Arginase, PGIF, Type I Arginase

Entrez Gene IDs

383 (Human); 11846 (Mouse); 29221 (Rat)

Gene Symbol

ARG1

UniProt

P05089

Additional Arginase 1/ARG1 Products

Product Documents for Recombinant Human Arginase 1/ARG1 Protein, CF

Product Datasheets

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Product Specific Notices for Recombinant Human Arginase 1/ARG1 Protein, CF

For research use only

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