Recombinant Human Arylsulfatase B/ARSB Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 4415-SU
Key Product Details
Product Specifications
Source
Chinese Hamster Ovary cell line, CHO-derived human Arylsulfatase B/ARSB protein
Ser37-Met533, with a C-terminal 10-His tag
Ser37-Met533, with a C-terminal 10-His tag
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Ser37
Predicted Molecular Mass
57 kDa
SDS-PAGE
80 kDa, reducing conditions
Activity
Measured by its ability to hydrolyze the substrate 4-Nitrocatechol Sulfate (PNCS).
The specific activity is >3,000 pmol/min/µg, as measured under the described conditions.
The specific activity is >3,000 pmol/min/µg, as measured under the described conditions.
Formulation, Preparation and Storage
4415-SU
| Formulation | Supplied as a 0.2 μm filtered solution in Tris and NaCl. |
| Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: Arylsulfatase B/ARSB
ARSB is a lysosomal enzyme of the sulfatase family that functions as an N-acetylgalactosamine-4-sulfatase (1). The enzyme is also active against chondroitin sulfate and dermatan sulfate (2). Deficiencies in ARSB activity are responsible for the lysosomal storage disease mucopolysaccharidosis Type VI, also known as Maroteaux-Lamy Syndrome (3).
References
- Peters, C. et al. (1990) J. Biol. Chem. 265:3374.
- O’Brien, J.S. et al. (1974) Biochem. Biophys. Res. Commun. 60:1170.
- Wicker, G. et al. (1991) J. Biol. Chem. 266:21386.
Alternate Names
ARSB, ASB, N-acetylgalactosamine-4-sulfatase
Gene Symbol
ARSB
UniProt
Additional Arylsulfatase B/ARSB Products
Product Documents for Recombinant Human Arylsulfatase B/ARSB Protein, CF
Product Specific Notices for Recombinant Human Arylsulfatase B/ARSB Protein, CF
For research use only
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