Recombinant Human ASIP/Agouti Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 9094-AG

R&D Systems, part of Bio-Techne
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9094-AG-050
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Key Product Details

Source

HEK293

Accession #

P42127

Conjugate

Unconjugated

Applications

Bioactivity

View all ASIP Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human ASIP protein
His23-Cys132

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

His23

Predicted Molecular Mass

12 kDa

SDS-PAGE

18-23 kDa, reducing conditions

Activity

Measured by its ability to inhibit alpha-MSH induced eumelanin production in B16F1 mouse melanoma cells.
The ED50 for this effect is 0.8-4 μg/mL.

Scientific Data Images for Recombinant Human ASIP/Agouti Protein, CF

Recombinant Human ASIP/Agouti Protein Bioactivity

Recombinant Human ASIP/Agouti Protein Bioactivity

Recombinant Human ASIP/Agouti (Catalog # 9094-AG) inhibits alpha-MSH induced eumelanin production in B16F1 mouse melanoma cells. The ED50 for this effect is 0.8-4 μg/mL.

Formulation, Preparation and Storage

9094-AG
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 400 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ASIP

Agouti, also known as Agouti Signaling Protein (ASIP), is a 132 amino peptide that regulates hair pigmentation in mice and adipocyte metabolism in humans (1). Mature human Agouti shares 78% and 76% amino acid sequence identity with mouse and rat Agouti, respectively (2, 3). Human Agouti is primarily expressed in adipose tissue, whereas its adipose expression in mice is only induced by mutations in the Agouti promoter (4, 5). Agouti is also expressed in the pancreas where it enhances the production of insulin by beta cells (6). Agouti functions as a competitive antagonist at several melanocortin receptors. It blocks ACTH binding to MC1R and MSH binding to MC1R, MC3R, and MC4R (7-9). It blocks MSH induced effects on melanocytes including proliferation and melanin production (3, 7, 8).

References

  1. Voisey, J. and A. van Daal (2002) Pigment Cell Res. 15:10.
  2. Kwon, H.Y. et al. (1994) Proc. Natl. Acad. Sci. USA 91:9760.
  3. Wilson, B.D. et al. (1995) Hum. Mol. Genet. 4:223.
  4. Mynatt, R.L. et al. (1997) Proc. Natl. Acad. Sci. USA 94:919.
  5. Mynatt, R.L. and J.M. Stephens (2001) Am. J. Physiol. Cell Physiol. 280:C954.
  6. Xue, B.Z. et al. (1999) Physiol. Genomics 1:11.
  7. Lu, D. et al. (1994) Nature 371:799.
  8. Suzuki, I. et al. (1997) J. Invest. Dermatol. 108:838.
  9. Kiefer, L.L. et al. (1997) Biochemistry 36:2084.

Long Name

Agouti Signaling Protein

Alternate Names

AGSW, AGTI, AGTIL, SHEP9

Entrez Gene IDs

434 (Human); 50518 (Mouse); 24152 (Rat)

Gene Symbol

ASIP

UniProt

P42127

Additional ASIP Products

Product Documents for Recombinant Human ASIP/Agouti Protein, CF

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Product Specific Notices for Recombinant Human ASIP/Agouti Protein, CF

For research use only

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