Recombinant Human Axl Fc Chimera Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 154-AL
Key Product Details
Source
NS0
Accession #
Structure / Form
Disulfide-linked homodimer
Conjugate
Unconjugated
Applications
Binding Activity
Product Specifications
Source
Mouse myeloma cell line, NS0-derived human Axl protein
Human Axl (Glu33-Pro440) Accession # AAA61243 |
DIEGRMD | Human IgG1 (Pro100-Lys330) |
6-His tag |
N-terminus | C-terminus |
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.1 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Glu33
Predicted Molecular Mass
71.7 kDa (monomer)
SDS-PAGE
100-110 kDa, reducing conditions
Activity
Measured by its binding ability in a functional ELISA.
When Recombinant Human Axl Fc Chimera is immobilized at 2 µg/mL (100 µL/well), the concentration of Recombinant Human Gas6 (Catalog # 885-GS) that produces 50% of the optimal binding response is approximately 2.5‑15 ng/mL.
When Recombinant Human Axl Fc Chimera is immobilized at 2 µg/mL (100 µL/well), the concentration of Recombinant Human Gas6 (Catalog # 885-GS) that produces 50% of the optimal binding response is approximately 2.5‑15 ng/mL.
Reviewed Applications
Read 1 review rated 4 using 154-AL in the following applications:
Formulation, Preparation and Storage
154-AL
Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
Reconstitution |
Reconstitute at 100 μg/mL in sterile PBS.
|
Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: Axl
References
- Linger, R.M.A. et al. (2011) Adv. Cancer Res. 100:35.
- O’Bryan, J.P. et al. (1991) Mol. Cell. Biol. 11:5016.
- Nagata, K. et al. (1996) J. Biol. Chem. 22:30022.
- Cosemans, J.M.E.M. et al. (2010) J. Thromb. Haemost. 8:1797.
- Seitz, H.M. et al. (2007) J. Immunol. 178:5635.
- Park, I.-K. et al. (2009) Blood 113:2470.
- Holland, S. et al. (2005) Cancer Res. 65:9294.
- Rankin, E.B. et al. (2010) Cancer Res. 70:7570.
- Korshunov, V.A. et al. (2006) Circ. Res. 98:1446.
- Morizono, K. et al. (2011) Cell Host Microbe 9:286.
- O’Bryan, J.P. et al. (1995) J. Biol. Chem. 270:551.
- Ekman, C. et al. (2010) J. Thromb. Haemost. 8:838.
Long Name
Axl Receptor Tyrosine Kinase
Alternate Names
AI323647, ARK, JTK11, Tyro7, UFO
Gene Symbol
AXL
UniProt
Additional Axl Products
Product Documents for Recombinant Human Axl Fc Chimera Protein, CF
Product Specific Notices for Recombinant Human Axl Fc Chimera Protein, CF
For research use only
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