Recombinant Human B3GAT3 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 6808-GT

R&D Systems, part of Bio-Techne
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6808-GT-020
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Key Product Details

Source

E. coli

Accession #

O94766

Conjugate

Unconjugated

Applications

Enzyme Activity

View all beta-1,3-Glucuronyltransferase 3/B3GAT3 Proteins and Enzymes »

Product Specifications

Source

E. coli-derived human beta-1,3-Glucuronyltransferase 3/B3GAT3 protein
Glu72-Val335, with an N-terminal Met and 6-His tag

Purity

>80%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 μg per lane.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Met

Predicted Molecular Mass

30 kDa

SDS-PAGE

30-35 kDa, reducing conditions

Activity

Measured by its ability to hydrolyze UDP-GlcA.
The specific activity is >1 pmol/min/μg, as measured under the described conditions.

Formulation, Preparation and Storage

6808-GT
Formulation Supplied as a 0.2 μm filtered solution in Tris and NaCl.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.

Background: beta-1,3-Glucuronyltransferase 3/B3GAT3

B3GAT3 is an essential enzyme involved in the synthesis of the linkage region of heparan sulfate and chondroitin sulfate proteoglycans (1). It transfers a glucuronic acid moiety from the uridine diphosphate-glucuronic acid (UDP-GlcA) to the trisaccharide Gal beta1-3Gal beta1-4Xyl covalently bound to a Ser residue at the glycosaminylglycan attachment site of proteoglycans. B3GAT3 knockout mice are embryonic lethal before the 8-cell stage due to failed cytokinesis, suggesting critical roles for chondroitin sulfate and heparan sulfate in embryonic cell division (2). Unlike the broad substrate specificity exhibited by B3GAT1, B3GAT3 shows strict specificity for Gal beta1-3Gal beta1 (3). The crystal structure shows the enzyme is an alpha/ beta protein with two subdomains that constitute the donor and acceptor binding sites with the active site lying in the cleft between the two subdomains (4). Like most known glycosyltransferases, B3GAT3 is a type II Golgi-resident transmembrane protein with a short N‑terminal cytoplasmic domain and a single-pass transmembrane domain followed by an enzymatic domain in the lumen of Golgi apparatus. In the current assay, hydrolase activity against UDP-GlcA was measured using a phosphatase-coupled method (5).

References

  1. Kitagawa, H. et al. (1998) J. Biol. Chem. 273:6615.
  2. Isumikawa, T. et al. (1998) J. Biol. Chem. 285:12190.
  3. Fondeur-Gelinotte, M. et al. (2007) Glycobiology 17:857.
  4. Pedersen, L.C. et al. (2000) J. Biol. Chem. 275:34580.
  5. Wu, Z.L, et al. (2011) Glycobiology 21:727.

Long Name

UDP-GlcUA:Gal beta-1,3-Gal-R Glucuronyltransferase

Alternate Names

GlcAT-I, GLCATI, GlcUAT-I

Entrez Gene IDs

26229 (Human); 72727 (Mouse); 293722 (Rat)

Gene Symbol

B3GAT3

UniProt

O94766

Additional beta-1,3-Glucuronyltransferase 3/B3GAT3 Products

Product Documents for Recombinant Human B3GAT3 Protein, CF

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Product Specific Notices for Recombinant Human B3GAT3 Protein, CF

For research use only

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