Recombinant Human B3GNT2 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 3960-GT

R&D Systems, part of Bio-Techne
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Key Product Details

Learn more about Fluorescent Glycan Labeling and Detection

Source

NS0

Accession #

Q9NY97

Conjugate

Unconjugated

Applications

Enzyme Activity

View all Beta-1,3-N-Acetylglucosaminyltransferase 2/B3GNT2 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human Beta-1,3-N-Acetylglucosaminyltransferase 2/B3GNT2 protein
Lys29-Cys397, with N-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

His

Predicted Molecular Mass

44 kDa

SDS-PAGE

63-90 kDa, reducing conditions

Activity

Measured by its ability to transfer GlcNAc from UDP-GlcNAc to N-Acetyl-D-Lactosamine.
The specific activity is > 50 pmol/min/μg, as measured under the described conditions.

Formulation, Preparation and Storage

3960-GT
Formulation Supplied as a 0.2 μm filtered solution in Tris and NaCl.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.

Background: Beta-1,3-N-Acetylglucosaminyltransferase 2/B3GNT2

beta1,3-Linked GlcNAc residues are present in the backbone of various biologically important glycans which are involved in many essential biological functions such as keratan sulfate synthesis in corneal tissue (1). The addition of such residues are catalyzed by a family of beta1,3-N-acetylglucosaminyltransferases, that includes at least eight members (1-5). All of them are type II Golgi resident transmembrane proteins and have high homology to the beta1,3-galactosyltransferase family. beta1,3‑N‑acetylglucosaminyltransferases 2 or beta3GNT2 prefers the substrate lacto-N-neotetraose and is involved in the biosynthesis of poly‑N‑acetyllactosamine chains (6). The enzymatic activity of the recombinant protein was determined using a phosphatase-coupled assay (7).

References

  1. Seko, A. and Yamashita, K. (2005) Glycobiology 15:943.
  2. Kataoka, K. and Huh, N.-H. (2002) Biochem. Biophys. Res. Commun. 294:843.
  3. Iwai,T. et al. (2002) J. Biol. Chem. 277:12802.

  4. Togayachi, A. et al. (2001) J. Biol. Chem. 276:22032.

  5. Sasaki, K. et al. (1997) Proc. Natl. Acad. Sci. USA 94:14294.

  6. Shiraishi, N. et al. (2001) J. Biol. Chem. 276:3498.
  7. Wu, Z.L. et al. (2011) Glycobiology 21:727.

Long Name

UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyl-transferase 1

Alternate Names

B3Galt6, Beta3gnt1

Entrez Gene IDs

10678 (Human); 53625 (Mouse)

Gene Symbol

B3GNT2

UniProt

Q9NY97

Additional Beta-1,3-N-Acetylglucosaminyltransferase 2/B3GNT2 Products

Product Documents for Recombinant Human B3GNT2 Protein, CF

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Human B3GNT2 Protein, CF

For research use only

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