Recombinant Human B7-H7/HHLA2 Fc Chimera Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 8084-B7

R&D Systems, part of Bio-Techne
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8084-B7-050
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Key Product Details

Source

HEK293

Accession #

Q9UM44

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all B7-H7/HHLA2 Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human B7-H7/HHLA2 protein
Human B7-H7/HHLA2
(Ile23-Asn344)
Accession # Q9UM44		 IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ile23

Predicted Molecular Mass

64 kDa (monomer)

SDS-PAGE

90-100 kDa, reducing conditions

Activity

Measured by its ability to inhibit anti-CD3 antibody induced IL-2 secretion in human T lymphocytes.
The ED50 for this effect is 0.075-0.75 μg/mL.

Scientific Data Images for Recombinant Human B7-H7/HHLA2 Fc Chimera Protein, CF

Recombinant Human B7-H7/HHLA2 Fc Chimera Protein Bioactivity

Recombinant Human B7-H7/HHLA2 Fc Chimera Protein Bioactivity

Recombinant Human B7-H7/HHLA2 Fc Chimera (Catalog # 8084-B7) inhibits anti-CD3 antibody induced IL-2 secretion in human T lymphocytes. The ED50 for this effect is 0.075-0.75 µg/mL in the presence of Goat Anti-Human IgG Fc Polyclonal Antibody (Catalog # G-102-C).

Formulation, Preparation and Storage

8084-B7
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 100 μg/mL in PBS.

Reconstitution Buffer Available:
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Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: B7-H7/HHLA2

B7-H7, also known as HHLA2 (HERV-H LTR-associating 2), is a member of the B7 family of immune regulatory proteins (1, 2). Mature human B7-H7 consists of a 322 amino acid (aa) extracellular domain (ECD) with three immunoglobulin-like domains, a 21 aa transmembrane segment, and a 49 aa cytoplasmic domain (3-5). B7-H7 is constitutively expressed on monocytes and is up-regulated by LPS and IFN-gamma stimulation. It is expressed on LPS/IFN-gamma treated B cells but not on resting B cells (5). B7-H7 binds to cell surface determinants on resting and mature T cells, B cells, and monocytes as well as on immature and mature dendritic cells (5). Soluble B7-H7 inhibits the proliferation of activated CD4+ and CD8+ T cells and their production of IFN-gamma, TNF-alpha, IL-5, IL-10, IL-13, IL-17A, and IL-22 (5).

References

  1. Zou, W. and L. Chen (2008) Nat. Rev. Immunol. 8:467.
  2. Bour-Jordan, H. et al. (2011) Immunol. Rev. 241:180.
  3. Mager, D.L. et al. (1999) Genomics 59:255.
  4. Flajnik, M.M. et al. (2012) Immunogenetics 64:571.
  5. Zhao, R. et al. (2013) Proc. Natl. Acad. Sci. USA 110:9879.

Long Name

B7 Homolog 7

Alternate Names

B7H7, HHLA2

Entrez Gene IDs

11148 (Human); 102137704 (Cynomolgus Monkey)

Gene Symbol

HHLA2

UniProt

Q9UM44

Additional B7-H7/HHLA2 Products

Product Documents for Recombinant Human B7-H7/HHLA2 Fc Chimera Protein, CF

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Human B7-H7/HHLA2 Fc Chimera Protein, CF

For research use only

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