Recombinant Human BAFF/BLyS/TNFSF13B His-tag Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 11403-BF

(HEK293-expressed)
R&D Systems, part of Bio-Techne
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11403-BF-050
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Key Product Details

Source

HEK293

Accession #

Q9Y275.1

Conjugate

Unconjugated

Applications

Bioactivity

View all BAFF/BLyS/TNFSF13B Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human BAFF/BLyS/TNFSF13B protein
MHHHHHHHHHH GGGSGGGSGGGS IEGR Human BAFF
(Ala134-Leu285)
Accession # Q9Y275.1
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Met

Predicted Molecular Mass

20 kDa

SDS-PAGE

20-28 kDa, under reducing conditions.

Activity

Measured in a cell proliferation assay using anti-IgM stimulated mouse B cells.
The ED50 for this effect is 0.300-3.60 ng/mL.

Scientific Data Images for Recombinant Human BAFF/BLyS/TNFSF13B His-tag Protein, CF

Recombinant Human BAFF/BLyS/TNFSF13B His-tag Protein Bioactivity.

Recombinant Human BAFF/BLyS/TNFSF13B His-tag (Catalog # 11403-BF) induces proliferation of anti-IgM stimulated mouse B cells. The ED50 for this effect is 0.300-3.60 ng/mL.

Formulation, Preparation and Storage

11403-BF
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: BAFF/BLyS/TNFSF13B

B-cell activating factor (BAFF), also known as BLyS, TALL-1, THANK, and TNFSF13B, is a 32 kDa transmembrane glycoprotein in the TNF ligand superfamily. It is involved in multiple aspects of immune system regulation, particularly towards B cells (1, 2). Mature human BAFF consists of a 46 amino acid (aa) cytoplasmic domain, a 21 aa transmembrane segment, and a 218 aa extracellular domain (ECD) with a stalk region and one TNF-like domain (3, 4). Within aa 134-285 of the ECD, human BAFF shares 72% aa sequence identity with mouse BAFF. It can be expressed as a homo-oligomer or as a heteromer in association with the related TNFSF member APRIL (4, 5). A 18 kDa fragment containing the TNF-like domain can be released by proteolysis between Arg133 and Ala134 (4). Soluble BAFF is stored intracellularly in neutrophils and released upon inflammatory stimulation (6). Alternative splicing generates an isoform termed deltaBAFF that lacks 19 aa between the proteolytic cleavage site and the TNF-like domain. deltaBAFF can form heteromers with BAFF and negatively regulates BAFF function (7). BAFF is produced by many hematopoietic cell types including monocytes, macrophages, neutrophils, dendritic cells, and T cells and also by adipocytes (1, 2, 8). Both BAFF and APRIL are functional ligands for the TNF receptor superfamily members BCMA and TACI, and BAFF additionally binds and signals through BAFF R (9, 10). All three receptors are primarily expressed by B cells (10). BAFF plays a critical role in the development and survival of B lineage cells (2, 11, 12). Mice that overexpress BAFF exhibit elevated B cell numbers, increased formation and size of germinal centers, and symptoms of autoimmunity (13). Soluble BAFF is elevated in B cell malignancies, autoimmunity, and other immune disorders (1). In addition, BAFF co‑stimulates T cell activation, promotes a Th1 biased immune response, and promotes the expansion of Treg cells (14‑16). BAFF also promotes monocyte survival, proinflammatory cytokine secretion, and differentiation to macrophages (17).

References

  1. Lied, G.A. and A. Berstad (2011) Scand. J. Immunol. 73:1.
  2. Mackay, F. et al. (2010) Immunol. Rev. 237:205.
  3. Moore, P.A. et al. (1999) Science 285:260.
  4. Schneider, P. et al. (1999) J. Exp. Med. 189:1747.
  5. Roschke, V. et al. (2002) J. Immunol. 169:4314.
  6. Scapini, P. et al. (2003) J. Exp. Med. 197:297.
  7. Gavin, A.L. et al. (2003) J. Biol. Chem. 278:38220.
  8. Alexaki, V.-I. et al. (2009) J. Immunol. 183:5948.
  9. Yu, G. et al. (2000) Nat. Immunol. 1:252.
  10. Thompson, J.S. et al. (2001) Science 293:2108.
  11. Schiemann, B. et al. (2001) Science 293:2111.
  12. Litinskiy, M.B. et al. (2002) Nat. Immunol. 3:822.
  13. Batten, M. et al. (2000) J. Exp. Med. 192:1453.
  14. Huard, B. et al. (2001) J. Immunol. 167:6225.
  15. Sutherland, A.P.R. et al. (2005) J. Immunol. 174:5537.
  16. Walters, S. et al. (2009) J. Immunol. 182:793.
  17. Chang, S.K. et al. (2006) Blood 108:2687.

Long Name

B cell Activating Factor

Alternate Names

BLyS, CD257, TALL1, THANK, TNFSF13B, ZTNF4

Entrez Gene IDs

10673 (Human); 24099 (Mouse); 498666 (Rat); 102123214 (Cynomolgus Monkey)

Gene Symbol

TNFSF13B

UniProt

Q9Y275.1

Additional BAFF/BLyS/TNFSF13B Products

Product Documents for Recombinant Human BAFF/BLyS/TNFSF13B His-tag Protein, CF

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Product Specific Notices for Recombinant Human BAFF/BLyS/TNFSF13B His-tag Protein, CF

For research use only

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