Recombinant Human BAI3 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 9106-BA

R&D Systems, part of Bio-Techne
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9106-BA-050
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Key Product Details

Source

HEK293

Accession #

NP_001695

Conjugate

Unconjugated

Applications

Bioactivity

View all BAI3 Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human BAI3 protein
Ala25-Thr880, with C-terminal 6-His tag

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ala25

Predicted Molecular Mass

97 kDa

SDS-PAGE

109-132 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human C1qL3 (Catalog # 9115-TN) is immobilized at 1 μg/mL, 100 μL/well, the concentration of Recombinant Human BAI3 that produces 50% of the optimal binding response is approximately 0.3-1.8 μg/mL.

Scientific Data Images for Recombinant Human BAI3 Protein, CF

Recombinant Human BAI3 Protein Bioactivity

Recombinant Human BAI3 Protein Bioactivity

When Recombinant Human C1qL3 (Catlog # 9115-TN) is coated at 1 µg/mL (100 μL/well), Recombinant Human BAI3 (Catlog # 9106-BA) binds with a typical ED50 of 0.3-1.8 μg/mL.

Formulation, Preparation and Storage

9106-BA
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution
Reconstitute at 1 mg/mL in PBS.

Reconstitution Buffer Available:
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Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: BAI3

Brain angiogenesis inhibitor 3 (BAI3) is an approximately 180 kDa adhesion GPCR that plays a role in neuronal synapse formation and maintenance. Mature human BAI3 consists of an 856 amino acid (aa) N-terminal extracellular domain (ECD) with one CUB domain, four TSP1 domains, and a GPS protease sensitive linker, followed by a region with seven transmembrane segments and a 376 aa C-terminal cytoplasmic domain (1, 2). Within the N-terminal ECD, human BAI3 shares 98% and 97% aa sequence identity with mouse and rat BAI3, respectively. BAI3 is primarily expressed post-synaptically in the cerebral cortex and on cerebellar Purkinje cells (1-5), and it binds to the TNF-like proteins C1qL1, 2, 3, and 4 (6). BAI3 interaction with C1qL1 is required for the formation and maintenance of excitatory synapses between climbing fibers and parallel fibers with Purkinje cells (4, 5). BAI3 ligation can also reduce excitatory synaptic density in hippocampal neurons, decrease dendrite arborization, and promote axon pruning in climbing fibers (3, 4, 6). It is additionally expressed in developing muscle where it is required for myoblast fusion (7).

References

  1. Shiratsuchi, T. et al. (1997) Cytogenet. Cell Genet. 79:103.
  2. Kee, H.J. et al. (2004) FEBS Lett. 569:307.
  3. Lanoue, V. et al. (2013) Mol. Psychiatry 18:943.
  4. Kakegawa, W. et al. (2015) Neuron 85:316.
  5. Sigoillot, S.M. et al. (2015) Cell Rep. 10:820.
  6. Bolliger, M.F. et al. (2011) Proc. Natl. Acad. Sci. USA 108:2534.
  7. Hamoud, N. et al. (2014) Proc. Natl. Acad. Sci. USA 111:3745.

Long Name

Brain-specific Angiogenesis Inhibitor 3

Alternate Names

brain-specific angiogenesis inhibitor 3, dJ91B17.1 (brain-specific angiogenesis inhibitor 3), KIAA0550MGC133100

Entrez Gene IDs

577 (Human); 210933 (Mouse); 301309 (Rat)

Gene Symbol

ADGRB3

UniProt

NP_001695

Additional BAI3 Products

Product Documents for Recombinant Human BAI3 Protein, CF

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Product Specific Notices for Recombinant Human BAI3 Protein, CF

For research use only

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