Recombinant Human BAMBI/NMA Fc Chimera Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 921-BM

R&D Systems, part of Bio-Techne
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921-BM-050
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Key Product Details

Source

NS0

Accession #

Q13145

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all BAMBI/NMA Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human BAMBI/NMA protein
Human BAMBI/NMA
(Glu27-Ala152)
Accession # Q13145		 DIEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>90%, by SDS-PAGE with silver staining, under reducing conditions.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Glu27

Predicted Molecular Mass

40.5 kDa (monomer)

SDS-PAGE

45-48 kDa, reducing conditions

Activity

Measured by its ability to induce cell death using Mv1Lu mink lung epithelial cells.
The ED50 for this effect is 1-6 μg/mL.

Formulation, Preparation and Storage

921-BM
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 500 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: BAMBI/NMA

BAMBI (BMP and Activin membrane-bound inhibitor), also called NMA (non-metastatic gene A) is a type I transmembrane protein that shares sequence homology with the TGF‑ beta type I receptor family and functions as a decoy receptor (1). The 260 amino acid (aa) human BAMBI protein contains a 20 aa signal sequence, a 132 aa extracellular domain (ECD) with one potential N‑glycosylation site, a transmembrane domain and a cytoplasmic domain. The human BAMBI ECD shares 91%, 93%, 94%, 94%, 92%, 92% and 92% aa sequence identity with mouse, rat, canine, porcine, equine, ovine and bovine BAMBI, respectively. BAMBI differs from the
TGF-beta  RI family by having a short cytoplasmic region that lacks the kinase domain (1, 2). It competes with type I receptors and forms heterodimers with type II receptors (2). During development, BAMBI is prominent in gastrulation, neurulation, and development of teeth and bones, and is often co‑expressed with BMP family members (1, 2, 4, 6). It antagonizes Activin and TGF‑ beta signaling, and may increase the dynamic range of BMPs to aid in BMP gradient effectiveness (4, 6). In the adult, it modulates processes such as adipogenesis, response to arterial injury, and tumor/tumor stroma promotion by TGF-beta (1, 3, 7, 9‑11). Although it is not expressed in other normal skin cells, it is highly expressed in melanocytes and may be inversely correlated with metastatic potential in melanomas (3). BAMBI is induced by TGF‑ beta and Wnt signaling, and cooperates with inhibitory Smad6 and Smad7 to inhibit TGF‑ beta signaling (5). In contrast, it promotes Wnt signaling (8).

References

  1. Knight, C. et al. (2001) J. Dent. Res. 80:1895.
  2. Onichtchouk, D. et al. (1999) Nature 401:480.
  3. Degen, W.G. et al. (1996) Int. J. Cancer 65:460.
  4. Paulsen, M. et al. (2011) Proc. Natl. Acad. Sci. USA 108:10202.
  5. Yan, X. et al. (2009) J. Biol. Chem. 284:30097.
  6. Montero, J.A. et al. (2008) Dev. Biol. 321:343.
  7. Guillot, N. et al. (2013) PLoS ONE 8:e58550.
  8. Lin, Z. et al. (2008) J. Biol. Chem. 283:33053.
  9. Luo, X. et al. (2012) Diabetes 61:124.
  10. Shangguan, L. et al. (2012) Stem Cells 30:2810.
  11. Sekiya, T. et al. (2004) J. Biol. Chem. 279:6840.

Long Name

BMP and Activin Membrane-bound Inhibitor

Alternate Names

NMA

Entrez Gene IDs

25805 (Human); 68010 (Mouse)

Gene Symbol

BAMBI

UniProt

Q13145

Additional BAMBI/NMA Products

Product Documents for Recombinant Human BAMBI/NMA Fc Chimera Protein, CF

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Human BAMBI/NMA Fc Chimera Protein, CF

For research use only

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