Recombinant Human beta-Glucuronidase/GUSB Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 6144-GH

R&D Systems, part of Bio-Techne
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6144-GH-020
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Key Product Details

Source

NS0

Accession #

AAA52561

Conjugate

Unconjugated

Applications

Enzyme Activity

View all beta-Glucuronidase/GUSB Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human beta-Glucuronidase/GUSB protein
Met1-Thr651, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Leu23

Predicted Molecular Mass

73 kDa

SDS-PAGE

75-85 kDa, reducing conditions

Activity

Measured by its ability to hydrolyze 4-methylumbelliferyl-beta -D-glucuronide.
The specific activity is >4,000 pmol/min/μg, as measured under the described conditions.

Formulation, Preparation and Storage

6144-GH
Formulation Supplied as a 0.2 μm filtered solution in Tris and NaCl.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: beta-Glucuronidase/GUSB

Human beta-Glucuronidase (EC 3.2.1.31) encoded by the GUSB gene is a lysosomal hydrolase involved in the stepwise degradation of glucuronic acid-containing glycosaminoglycans that include heparan sulfate, chondroitin sulfate and hyaluronan (1). The enzyme is only active on the glucuronic acid of the non-reducing end. The native protein has been reported as a tetrameric glycoprotein composed of identical subunits (1, 2). Mutations in the GUSB gene are linked to mucopolysaccharidosis type VII (3). Accumulation of partially degraded glycosaminoglycans, with glucuronic acid residues at the non-reducing termini, are usually found in the lysosomes of patients with the disease (3). It has also been reported that this enzyme may contribute to the depletion of chondroitin from cartilage and thereby facilitate the damage of joints in rheumatoid arthritis (4).

References

  1. Shipley, J.M. et al. (1993) Am. J. Hum. Genet. 52:517.
  2. Oshima, A, et al. (1987) Proc. Natl. Acad. Sci. USA 84:685.
  3. Bell, C.E. Jr. et al. (1977) J. Clin. Invest. 59:97.
  4. Ortutay, Z. et al. (2003) Arthritis Rheum. 48:2163.

Alternate Names

Beta-G1, MPS7

Entrez Gene IDs

2990 (Human)

Gene Symbol

GUSB

UniProt

AAA52561

Additional beta-Glucuronidase/GUSB Products

Product Documents for Recombinant Human beta-Glucuronidase/GUSB Protein, CF

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Human beta-Glucuronidase/GUSB Protein, CF

For research use only

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