Recombinant Human Biliverdin Reductase B/BLVRB Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 6568-BR

R&D Systems, part of Bio-Techne
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6568-BR-020
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Key Product Details

Source

E. coli

Accession #

P30043

Conjugate

Unconjugated

Applications

Enzyme Activity

View all Biliverdin Reductase B/BLVRB Proteins and Enzymes »

Product Specifications

Source

E. coli-derived human Biliverdin Reductase B/BLVRB protein
Ala2-Gln206, with an N-terminal Met and 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

N-terminus confirmed by detection of His tag using Western analysis.

Predicted Molecular Mass

23 kDa

SDS-PAGE

23-25 kDa, reducing conditions

Activity

Measured by the reduction of riboflavin 5'-monophosphate (FMN) using NADPH as the cofactor.
The specific activity is >225 pmol/min/μg, as measured under the described conditions.

Formulation, Preparation and Storage

6568-BR
Formulation Supplied as a 0.2 μm filtered solution in Tris, NaCl, Brij and Glycerol.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: Biliverdin Reductase B/BLVRB

Clearance of heme in mammals is a two-step process starting with conversion of heme to biliverdin by heme oxygenase, followed by reduction of biliverdin to bilirubin by bilivredin reductase. Biliverdin IX b reductase (BLVRB) converts the  beta isomer of biliverdin IX to bilirubin IX b, which constitutes 87% of total bilirubin in fetal bile. Therefore BLVRB is especially important for fetal heme metabolism and clearance (1). BLVRB is a cytoplasmic enzyme expressed at high levels in erythrocytes and liver, but is present in other tissues (2). The enzyme is identical to flavin reductase, which is an oxidoreductase that catalyses the NADPH-dependent reduction for a variety of flavins, such as riboflavin, FAD or FMN and met‑hemoglobin (3, 4). BLVRB is structurally distinct from BLVRA. In contrast to BLVRA, which prefers the biliverdin alpha isomer but could also use the  beta isomer as substrate, BLVRB is specific for the beta isomer (5, 6).

References

  1. Pereira, P.J. et al. (2001) Nat. Struct. Biol. 8:215.
  2. Chikuba, K. et al. (1994) Biochem. Biophys. Res. Commun. 198:1170.
  3. Shalloe, F. et al. (1996) Biochem. J. 316:385.
  4. Cunningham, O. et al. (2000) Biochem. J. 345:393.
  5. Yamaguchi, T. et al. (1994) J. Biol. Chem. 269:24343.
  6. Cunningham, O. et al. (2000) J. Biol. Chem. 275:19009.

Alternate Names

BLVRB, BVRB, FLR, GHBP

Entrez Gene IDs

645 (Human)

Gene Symbol

BLVRB

UniProt

P30043

Additional Biliverdin Reductase B/BLVRB Products

Product Documents for Recombinant Human Biliverdin Reductase B/BLVRB Protein, CF

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Product Specific Notices for Recombinant Human Biliverdin Reductase B/BLVRB Protein, CF

For research use only

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