Recombinant Human BMP-10 Protein Best Seller
R&D Systems, part of Bio-Techne | Catalog # 2926-BP
Key Product Details
Source
CHO
Accession #
Structure / Form
Disulfide-linked homodimer
Conjugate
Unconjugated
Applications
Bioactivity
Product Specifications
Source
Chinese Hamster Ovary cell line, CHO-derived human BMP-10 protein
Asn317-Arg424
Asn317-Arg424
Purity
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Asn317
Predicted Molecular Mass
12.2 kDa (monomer)
SDS-PAGE
13 kDa, reducing conditions
Activity
Measured by its ability to induce alkaline phosphatase production by MC3T3-E1 mouse preosteoblast cells.
The ED50 for this effect is 15-60 ng/mL.
The ED50 for this effect is 15-60 ng/mL.
Reviewed Applications
Read 1 review rated 4 using 2926-BP in the following applications:
Formulation, Preparation and Storage
Carrier Free
What does CF mean?CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
What formulation is right for me?In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
Carrier: 2926-BP
| Formulation | Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile 4 mM HCI containing at least 0.1% human or bovine serum albumin. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Carrier Free: 2926-BP/CF
| Formulation | Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile 4 mM HCI. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: BMP-10
non-glycosylated homodimers (4, 5). Mature human BMP-10 shares 98% amino acid sequence identity with mouse and rat BMP-10 and 49% - 63% with human BMP-9, GDF-5, -6, and -7. BMP-10 is critical for the proper development of the heart but is not expressed until after cardiac patterning or looping are completed (6-8). BMP-10 production appears at the onset of trabeculation and chamber formation and is restricted to the right atrium in the adult heart (6-8). Homozygous BMP-10 knockout mice die in utero due to arrested cardiac development (7). BMP-10 is required for the expression of the cardiogenic transcription factors NKX2.5 and MEF2C in developing myocardium and the growth of embryonic cardiomyocytes (7, 10). NKX2.5 itself negatively regulates BMP-10 expression in cardiac myocytes (10). Multiple human congenital heart defects result from mutations in NKX2.5 and require BMP-10 expression (10). In mice, genetic knockout of ErbB leads to a similar phenotype but appears not to involve BMP-10, and knockout of the calcium channel subunit FKBP12 induces BMP-10 over-expression (7). BMP-10 in the postnatal heart promotes increased cardiomyocyte and heart size (8). BMP-10 has been shown to signal through ALK-1, BMPR-IA, BMPR-IB, and BMPR-II in transfectants and non-cardiac cell lines (4, 5). A functional BMP-10 receptor in the heart has not yet been identified, although deletion of BMPR-IA causes similar cardiac morphogenetic abnormalities (11). In dermal endothelial cells, BMP-10 induces migration, proliferation, and gene expression typically associated with ALK-1 (5).
References
- Chen, D. et al. (2004) Growth Factors 22:233.
- Miyazono, K. et al. (2005) Cytokine Growth Factor Rev. 16:251.
- Schneider, M.D. et al. (2003) Cytokine Growth Factor Rev. 14:1.
- Mazerbourg, S. et al. (2005) J. Biol. Chem. 280:32122.
- David, L. et al. (2007) Blood 109:1953.
- Neuhaus, H. et al. (1999) Mech. Dev. 80:181.
- Chen, H. et al. (2004) Development 131:2219.
- Chen, H. et al. (2006) J. Biol. Chem. 281:27481.
- Srivastava, D. and E.N. Olson (2000) Nature 407:221.
- Pashmforoush, M. et al. (2004) Cell 117:373.
- Gaussin, V. et al. (2002) Proc. Natl. Acad. Sci. 99:2878.
Long Name
Bone Morphogenetic Protein 10
Alternate Names
BMP10
Gene Symbol
BMP10
UniProt
Additional BMP-10 Products
Product Documents for Recombinant Human BMP-10 Protein
Product Specific Notices for Recombinant Human BMP-10 Protein
For research use only
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