Recombinant Human BMP-2/BMP-6 Heterodimer Protein

R&D Systems, part of Bio-Techne | Catalog # 7145-BP

R&D Systems, part of Bio-Techne
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Carrier Free
7145-BP-010/CF
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With Carrier
7145-BP-010
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Key Product Details

Source

E. coli

Accession #

P12643 (BMP-2) & P22004 (BMP-6)

Structure / Form

Disulfide-linked heterodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all BMP-2/BMP-6 Heterodimer Proteins and Enzymes »

Product Specifications

Source

E. coli-derived human BMP-2/BMP-6 Heterodimer protein
Human BMP-2
(Ala284 - Arg396), with an N-terminal Met
Accession # P12643
Human BMP-6
(Gln382 - His513), with an N-terminal Met
Accession # P22004
N-terminus C-terminus

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ala284 (BMP-2) & Met (BMP-6)

Predicted Molecular Mass

12.8 kDa (BMP-2) & 15 kDa (BMP-6)

SDS-PAGE

11 kDa & 14 kDa, reducing conditions

Activity

Measured by its ability to induce alkaline phosphatase production by ATDC5 mouse chondrogenic cells. Binnerts, M.E. et al. (2004) Biochem. Biophys. Res. Commun. 315(2):272.

The ED50 for this effect is 4-20 ng/mL.

Formulation, Preparation and Storage

Carrier Free
What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Carrier: 7145-BP
Formulation Lyophilized from a 0.2 μm filtered solution in HCl with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in 4 mM HCl containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Carrier Free: 7145-BP/CF
Formulation Lyophilized from a 0.2 μm filtered solution in HCl.
Reconstitution Reconstitute at 100 μg/mL in 4 mM HCl.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: BMP-2/BMP-6 Heterodimer

Bone Morphogenetic Protein 6 (BMP-6), also known as Vgr-1, and BMP-2 are members of the BMP family of structurally and functionally related proteins and represent a subfamily of the transforming growth factor beta (TGF-beta) superfamily. BMPs are involved in a wide range of processes including embryogenesis, tissue morphogenesis, cell differentiation and migration, and tumorigenesis. Cellular responses to BMPs are mediated by hetero‑oligomeric complexes of type I and type II serine/threonine kinase receptors (1 - 4). Human BMP-2 is synthesized as a 396 amino acid (aa) preproprotein that contains a 23 aa signal sequence, a 259 aa prosegment, and a 114 aa mature region (5). Human BMP-6 is synthesized as a 513 aa precursor protein that contains a 20 aa signal sequence, a 354 aa prosegment, and a 139 aa mature region (6). BMP prosegments are removed by proteolysis, enabling the glycosylated 18 kDa mature BMPs to form active disulfide-linked homodimers or heterodimers (1, 2). Mature human BMP-2 shares 100% aa sequence identity with mouse and rat BMP-2, and mature human BMP-6 shares 96% and 98% aa sequence identity with mouse and rat BMP-6, respectively. They share 48% aa sequence identity with each other. Both BMP-2 and BMP-6 induce osteogenic and chondrogenic differentiation in mesenchymal stem cells (4). Heterodimers of BMP-2 and BMP-6 show increased potency at inducing osteoblastic calcium deposition, chondrogenesis, and in vivo bone formation compared to either BMP-2 or BMP-6 homodimers (7, 8). BMP-2/6 heterodimers also show increased activity at inducing trophoectodermal and endodermal differentiation of embryonic stem cells compared to either homodimer (9).

References

  1. Chen, D. et al. (2004) Growth Factors 22:233.
  2. Bragdon, B. et al. (2010) Cell Signal. 23:609.
  3. Singh, A. and R.J. Morris (2010) Cytokine Growth Factor Rev. 21:299.
  4. Lavery, K. et al. (2008) J. Biol. Chem. 283:20948.
  5. Wozney, J. et al. (1988) Science 242:1528.
  6. Celeste, A.J. et al. (1990) Proc. Natl. Acad. Sci. 87:9843.
  7. Isaacs, M.J. et al. (2010) Mol. Endocrinol. 24:1469.
  8. Israel, D.I. et al. (1996) Growth Factors 13:291.
  9. Valera, E. et al. (2010) PLoS ONE 5:e11167.

Long Name

Bone Morphogenetic Protein 2/6

Entrez Gene IDs

650 (Human)

Gene Symbol

BMP2

UniProt

P12643 (BMP-2) & P22004 (BMP-6)

Additional BMP-2/BMP-6 Heterodimer Products

Product Documents for Recombinant Human BMP-2/BMP-6 Heterodimer Protein

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS 7145-BP (with carrier)
Download SDS 7145-BP/CF (carrier free)

Product Specific Notices for Recombinant Human BMP-2/BMP-6 Heterodimer Protein

For research use only

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