Recombinant Human BTLA Fc Chimera (aa 31-153) Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 9509-BT

R&D Systems, part of Bio-Techne
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Key Product Details

Source

HEK293

Accession #

Q7Z6A9-1

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all BTLA Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human BTLA protein
Human BTLA
(Lys31-Trp153)
Accession # Q7Z6A9-1		 IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Lys31

Predicted Molecular Mass

41 kDa

SDS-PAGE

52-61 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Mouse HVEM/TNFRSF14 Fc Chimera (Catalog # 2516-HV) is immobilized at 0.5 μg/mL, 100 μL/well, the concentration of Recombinant Human BTLA Fc Chimera that produces 50% of the optimal binding response is 15-90 ng/mL.

Scientific Data Images for Recombinant Human BTLA Fc Chimera (aa 31-153) Protein, CF

Recombinant Human BTLA Fc Chimera (aa 31-153) Protein Bioactivity

Recombinant Human BTLA Fc Chimera (aa 31-153) Protein Bioactivity

When Recombinant Mouse HVEM/TNFRSF14 Fc Chimera (Catalog # 2516-HV) is coated at 0.5 µg/mL (100 µL/well), Recombinant Human BTLA Fc Chimera (Catalog # 9509-BT) binds with an ED50 of 15-90 ng/mL.

Formulation, Preparation and Storage

9509-BT
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 500 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
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Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: BTLA

B- and T- lymphocyte attenuator (BTLA), also known as B- and T-lymphocyte-associated protein and CD272, is a type I transmembrane glycoprotein that belongs to the CD28 family of T cell co-stimulatory molecules (1-3). Mature human BTLA contains a 127 amino acid (aa) extracellular domain (ECD), a 21 aa transmembrane domain, and a 111 aa cytoplasmic domain. The ECD of human BTLA shares 42% and 44% aa identity with mouse and rat BTLA, respectively. Unlike other CD28 family members, the BTLA Ig domain in the ECD is of the I-type rather than V-type (4). BTLA is expressed on T cells, B cells, macrophages, dendritic cells and NK cells (5). BTLA is also unusual in its interaction with the TNF superfamily member HVEM rather than with B7 family ligands (6). Its expression is low in naïve T cells and increases during antigen-specific induction of anergy. BTLA apparently limits T cell numbers, since its deletion results in overproduction of T cells, especially CD8+ memory T cells that are hyper-responsive to TCR cross-linking (7). Under the control of ROR gammat and IL-7, BTLA regulates the homeostasis and inflammatory responses of gamma  deltaT cells (8). The binding of BTLA and HVEM does not preclude the concurrent binding of other HVEM ligands such as LIGHT or Lymphotoxin‑alpha (10).

References

  1. Murphy, K.M. et al. (2006) Nat. Rev. Immunol. 6:671.
  2. Croft, M. (2005) Trends. Immunol. 26:292.
  3. Watannabe, N. et al. (2003) Nat. Immunol. 4:670.
  4. Compaan, D.M. et al. (2005) J. Biol. Chem. 280:39553.
  5. Hurchia, M.A. et al. (2005) J. Immunol. 174:3377.
  6. Sedy, J. R. et al. (2005) Nat. Immunol. 6:90.
  7. Krieg, C. et al. (2007) Nat. Immunol. 8:162.
  8. Bekiaris, V. et al. (2013) Immunity 39:1082.
  9. Gavrieli, M. et al. (2003) Biochem. Biophys. Res. Commun. 312:1236.
  10. Cai G and Freeman GJ, (2009) Immunol Rev. 229:244

Long Name

B- And T-Lymphocyte Attenuator

Alternate Names

CD272

Entrez Gene IDs

151888 (Human); 208154 (Mouse); 102144877 (Cynomolgus Monkey)

Gene Symbol

BTLA

UniProt

Q7Z6A9-1

Additional BTLA Products

Product Documents for Recombinant Human BTLA Fc Chimera (aa 31-153) Protein, CF

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Product Specific Notices for Recombinant Human BTLA Fc Chimera (aa 31-153) Protein, CF

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