Chinese Hamster Ovary cell line, CHO-derived human C1GalT1 protein
Asp45-Pro363, with N-terminal 6-His tag (C1GalT1); Ile28-Asp318, with an N-terminal HA tag (C1GalT1C1)

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

His (C1GalT1) & Tyr (C1GalT1C1)

Predicted Molecular Mass

38 kDa (C1GalT1) & 35 kDa (C1GalT1C1)

SDS-PAGE

25-42 kDa, reducing conditions

Activity

Measured by its ability to transfer galactose from UDP-galactose to 4-nitrophenyl-alpha -D-galactosaminide.
The specific activity is >2,750 pmol/min/μg, as measured under the described conditions.

Formulation, Preparation and Storage

8659-GT

Formulation	Supplied as a 0.2 μm filtered solution in Tris and NaCl.
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20 to -70 °C as supplied. 3 months, -20 to -70 °C under sterile conditions after opening.

Background: C1GalT1

O-glycosylation is a ubiquitous post-translational modification of secreted and membrane bound proteins (1). The synthesis of mucin-type O-glycans is initiated by the addition of GalNAc to threonine or serine residues on proteins by polypeptide N-acetylgalactosaminyltransferases (GALNTs) (2). The GalNAc alpha1-O-Ser/Thr structure is then extended by other glycosyltransferases to form eight types of core O-glycans (3). Core 1 beta-3-galactosyltransferase (C1GalT1), in particular, synthesizes Gal-beta 1-3GalNAc alpha1-O-Ser/Thr, a precursor for all core 1 and core 2 based mucin-type O-glycans (4). These glycans play central roles in many processes, such as angiogenesis, thrombopoiesis, and kidney homeostasis (5). C1GalT1 forms a stable, non-covalent complex with Cosmc chaperone, C1GalT1C1, which is required for the full activity of C1GalT1 (6). Defective C1GalT1 causes a rare autoimmune disease called Tn syndrome (4) as well as susceptibility to IgA nephropathy (7). The recombinant C1GalT1 is co-purified with C1GalT1C1. The enzymatic activity of recombinant human C1GalT1 was determined using a phosphatase-coupled assay (8).

References

Gerken, T.A. et al. (2011) J. Biol. Chem. 286:14493.
Bergstrom, K.S.B. and Xia, L. (2013) Glycobiology 23:1026.
Brockhausen, I. et al. (1999) Biochim. Biophys. Acta. 1473:67.
Ju, T. and Cummings, R.D. (2005) Nature 437:1252.
Fukuda, M. et al. (2002) Biochim. Biophys. Acta. 1573:394.
Aryal, R.P. et al. (2012) J. Biol. Chem. 287:15317.
Pirulli, D. et al. (2009) J. Nephrol. 22:152.
Wu, Z.L. et al. (2011) Glycobiology 21:727.

Long Name

Core 1 Synthase, Glycoprotein-N-Acetylgalactosamine 3-Beta-Galactosyltransferase, 1

Alternate Names

B3Gal-T8, Core 1 Beta3-Gal-T1, Core 1 O-Glycan T-Synthase

Entrez Gene IDs

56913 (Human); 94192 (Mouse); 65044 (Rat)

Gene Symbol

C1GALT1

UniProt

Q9NS00 (C1GalT1) & Q96EU7 (C1GalT1C1)

Additional C1GalT1 Products

Product Documents for Recombinant Human C1GalT1/C1GalT1C1 Protein, CF

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Recombinant Human C1GalT1/C1GalT1C1 Protein, CF

For research use only

Citations
Reviews

Recombinant Human C1GalT1/C1GalT1C1 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 8659-GT

Key Product Details

Source

Accession #

Conjugate

Applications

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Product Specifications