Recombinant Human Cadherin-13 (CAD-13) Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 3264-CA

R&D Systems, part of Bio-Techne
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3264-CA-050
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Key Product Details

Source

NS0

Accession #

P55290

Conjugate

Unconjugated

Applications

Bioactivity

View all Cadherin-13 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human Cadherin-13 protein
Glu23-Ala692, with a C-terminal 6-His tag

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Glu23

Predicted Molecular Mass

74.5 kDa

SDS-PAGE

100-115 kDa, reducing conditions

Activity

Measured by the ability of the immobilized protein to inhibit the adhesion of HUVEC human umbilical vein endothelial cells.

When 5 x 104 cells/well are added to rhCadherin-13 coated plates, cell adhesion is inhibited in a dose dependent manner after 2 hours at 37 °C. The ED50 for this effect is 2-8 μg/mL.

Formulation, Preparation and Storage

3264-CA
Formulation Lyophilized from a 0.2 μm filtered solution in MES and NaCl.
Reconstitution
Reconstitute at 200 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Cadherin-13

Cadherin-13, also known as T-Cadherin and H‑Cadherin, is a GPI-anchored protein belonging to the Cadherin superfamily of calcium-dependent adhesion molecules. Cadherins are involved in multiple processes including embryonic development, cell migration, and maintenance of epithelial integrity (1, 2). Following removal of its C‑terminal propeptide, Cadherin-13 is expressed on the cell surface both with and without its N-terminal propeptide in species of approximately 120 kDa and 100 kDa, respectively (3). Human Cadherin-13 contains five tandem Cadherin repeats and shares 95% aa sequence identity with mouse and rat Cadherin-13 (4). It is most highly expressed in the circulatory and nervous systems, particularly on cardiac myocytes, vascular endothelial and smooth muscle cells, and neurons and astrocytes in the brain (3, 5 - 9). Cadherin-13 exerts a negative influence on neurite extension, and it is down‑regulated in neurons upon NGF stimulation (10, 11). Homotypic Cadherin-13 interactions promote intercellular adhesion which can be inhibited by its binding to LDL (12). Cadherin-13 also mediates the cardioprotective effects of Adiponectin by directly binding Adiponectin (selectively the hexameric and HMW forms) and trapping it on vascular endothelial cells and cardiomyocytes (6 ‑ 8). The role of Cadherin-13 in the vasculature is complex. When expressed on vascular endothelial cells it promotes angiogenesis, but when expressed on stromal cells it inhibits neovascularization (13, 14). Its down‑regulation on breast carcinoma cells and up‑regulation on the vasculature of various tumors limits tumor cell proliferation and angiogenesis but also enhances tumor progression (4, 8, 15).

References

  1. Pokutta, S. and W.I. Weis (2007) Annu. Rev. Cell Dev. Biol. 23:237.
  2. Philippova, M. et al. (2009) Cell. Signal. 21:1035.
  3. Stambolsky, D.V. et al. (1999) Biochim. Biophys. Acta 1416:155.
  4. Lee, S.W. (1996) Nat. Med. 2:776.
  5. Doyle, D.D. et al. (1998) J. Biol. Chem. 273:6937.
  6. Denzel, M.S. et al. (2010) J. Clin. Invest. 120:4342.
  7. Hug, C. et al. (2004) Proc. Natl. Acad. Sci. 101:10308.
  8. Hebbard, L.W. et al. (2008) Cancer Res. 68:1407.
  9. Takeuchi, T. et al. (2000) J. Neurochem. 74:1489.
  10. Fredette, B.J. et al. (1996) Development 122:3163.
  11. Bai, S. et al. (2007) J. Biol. Chem. 282:27171.
  12. Resink, T.J. et al. (1999) FEBS Lett. 463:29.
  13. Philippova, M. et al. (2006) Arterioscler. Thromb. Vasc. Biol. 26:2222.
  14. Rubina, K. et al. (2007) Angiogenesis 10:183.
  15. Wyder, L. et al. (2000) Cancer Res. 60:4682.

Alternate Names

Cadherin13, CDH13, H-Cadherin, T-Cadherin

Entrez Gene IDs

1012 (Human); 12554 (Mouse); 192248 (Rat)

Gene Symbol

CDH13

UniProt

P55290

Additional Cadherin-13 Products

Product Documents for Recombinant Human Cadherin-13 (CAD-13) Protein, CF

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Human Cadherin-13 (CAD-13) Protein, CF

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