Recombinant Human Cadherin-7 (CAD-7) Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 6774-CA

R&D Systems, part of Bio-Techne
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6774-CA-050
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Key Product Details

Source

CHO

Accession #

BAA87415

Conjugate

Unconjugated

Applications

Bioactivity

View all Cadherin-7 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human Cadherin-7 protein
Met1-Thr607, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ser48

Predicted Molecular Mass

62.2 kDa

SDS-PAGE

80 - 90 kDa, reducing conditions

Activity

Measured by the ability of the immobilized protein to support the adhesion of U-87 MG human glioblastoma/astrocytoma cells.

The ED50 for this effect is 1.0-4.0 μg/mL.

Optimal dilutions should be determined by each laboratory for each application.

Formulation, Preparation and Storage

6774-CA
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 200 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Cadherin-7

Cadherin-7 is an approximately 115 kDa type I transmembrane protein belonging to the Cadherin superfamily of calcium-dependent adhesion molecules. Cadherins are involved in multiple processes including embryonic development, cell migration, and maintenance of epithelial integrity (1). Human Cadherin-7 is synthesized with a 27 amino acid (aa) signal peptide and a 20 aa N-terminal propeptide. The mature cell surface-expressed protein consists of a 738 amino acid (aa) extracellular domain (ECD) that contains five Cadherin repeats, a 21 aa transmembrane segment, and a 157 aa cytoplasmic domain (2, 3). Within the ECD, human Cadherin-7 shares 99% and 97% aa sequence identity with mouse and rat Cadherin-7, respectively. Cadherin-7 interacts homotypically and heterotypically with Cadherin-14 and more weakly with Cadherins-6, -9, and -12 (3, 4). Cellular adhesion mediated by Cadherin-7 is more weak than that mediated by E- or N-Cadherin, although it can be strengthed by Fibronectin binding to Integrins on the same cell (5, 6). Cadherin-7 is localized to discrete regions of the developing nervous system. In chick and mouse, it is expressed in the basal plate of the neural tube, migrating cranial motoneurons, and migrating neural crest cells (4, 7, 8), lateral regions of the hindbrain and migrating Purkinje cell precursors (8, 9), striatum, parahippocampal areas, and somatosensory cortex of the forebrain (10 - 12), neural retina and cochlea (13, 14). Cadherin-7 interactions promote motor axon growth and inhibit axonal branching, whereas Cadherin-6B promotes branching during cranial motorneuron development (7). Cadherin-7 performs a similar function during limb bud development during which it participates in the migration and condensation of mesenchymal cells (15). Cadherin-7 is overexpressed on primary melanoma cells where it binds melanoma inhibitory activity (MIA), a secreted melanoma cell protein that promotes tumor progression (16).

References

  1. Pokutta, S. and W.I. Weis (2007) Annu. Rev. Cell Dev. Biol. 23:237.
  2. Kools, P. et al. (2000) Genomics 68:283.
  3. Shimoyama, Y. et al. (2000) Biochem. J. 349:159.
  4. Nakagawa, S. et al. (1995) Development 121:1321.
  5. Chu, Y.-S. et al. (2006) J. Biol. Chem. 281:2901.
  6. Martinez-Rico, C. et al. (2009) J. Cell Sci. 123:712.
  7. Barnes, S.H. et al. (2010) Development 137:805.
  8. Ju, M.J. et al. (2004) Neuroscience 128:785.
  9. Luo, J. et al. (2004) Mol. Cell. Neurosci. 25:138.
  10. Redies, C. et al. (2002) Brain Res. Bull. 57:489.
  11. Kovjanic, D. and C. Redies (2003) J. Comp. Neurol. 456:95.
  12. Krishna-K, K. et al. (2011) Neuroscience 175:37.
  13. Faulkner-Jones, B.E. et al. (1999) Mol. Cell. Neurosci. 14:1.
  14. Luo, J. et al. (2007) Dev. Dyn. 236:2331.
  15. Kim, D. et al. (2009) J. Cell. Physiol. 221:161.
  16. Winklmeier, A. et al. (2009) Cancer Sci. 100:261.

Alternate Names

CAD7, Cadherin7, CDH7, CDH7L1

Entrez Gene IDs

1005 (Human); 241201 (Mouse); 29162 (Rat)

Gene Symbol

CDH7

UniProt

BAA87415

Additional Cadherin-7 Products

Product Documents for Recombinant Human Cadherin-7 (CAD-7) Protein, CF

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Product Specific Notices for Recombinant Human Cadherin-7 (CAD-7) Protein, CF

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