Recombinant Human Carboxypeptidase A1/CPA1 Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 2856-ZN
Key Product Details
Source
NS0
Accession #
Structure / Form
Pro form
Conjugate
Unconjugated
Applications
Enzyme Activity
Product Specifications
Source
Mouse myeloma cell line, NS0-derived human Carboxypeptidase A1/CPA1 protein
Lys17-Tyr419, with a C-terminal 10-His tag
Lys17-Tyr419, with a C-terminal 10-His tag
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Lys17
Predicted Molecular Mass
47 kDa
SDS-PAGE
42 kDa, reducing conditions
Activity
Measured by its ability to cleave the colorimetric peptide substrate Ac-Phe-Thiaphe-OH in the presence of 5,5’Dithio-bis (2-nitrobenzoic acid) (DTNB). Edwards, K.M. et al. (1999) J. Biol. Chem. 274:30468.
The specific activity is >3,000 pmol/min/µg, as measured under the described conditions.
The specific activity is >3,000 pmol/min/µg, as measured under the described conditions.
Formulation, Preparation and Storage
2856-ZN
| Formulation | Supplied as a 0.2 μm filtered solution in Tris and NaCl. |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: Carboxypeptidase A1/CPA1
Carboxypeptidase A1 encoded by the CPA1 gene cleaves the C-terminal amide or ester bond of peptides that have a free C-terminal carboxyl group (1). It prefers the C-terminal residues with aromatic or branched aliphatic side chains including Phe, Tyr, Trp, Leu or Ile. It is important in the degradation of food proteins to produce essential amino acids such as Phe and Trp. The deduced amino acid sequence of human CPA1 consists of a signal peptide (residues 1 to 16), a pro region (residue 17 to 110), and a mature chain (residues 111 to 419). The purified recombinant human CPA1 corresponds to the pro form, which can be activated as described in Activity Assay Protocol.
References
- Auld, D.S. (2004) in Handbook of Proteolytic Enzymes (ed. Barrett, et al.) pp. 812, Academic Press, San Diego.
Alternate Names
CPA1
Gene Symbol
CPA1
UniProt
Additional Carboxypeptidase A1/CPA1 Products
Product Documents for Recombinant Human Carboxypeptidase A1/CPA1 Protein, CF
Product Specific Notices for Recombinant Human Carboxypeptidase A1/CPA1 Protein, CF
For research use only
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