Recombinant Human Carboxypeptidase A4/CPA4 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 5906-ZN

R&D Systems, part of Bio-Techne
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5906-ZN-010
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Key Product Details

Source

NS0

Accession #

Q9UI42

Structure / Form

Monomer

Conjugate

Unconjugated

Applications

Enzyme Activity

View all Carboxypeptidase A4/CPA4 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human Carboxypeptidase A4/CPA4 protein
Gly17-Tyr421, with a C-terminal 10-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Gly17

Predicted Molecular Mass

47 kDa

SDS-PAGE

48 kDa, reducing conditions

Activity

Measured by its ability to cleave the colorimetric peptide substrate Ac-Phe-Thiaphe-OH in the presence of 5,5’Dithio-bis (2-nitrobenzoic acid) (DTNB). Edwards, K.M. et al. (1999) J. Biol. Chem. 274:30468.
The specific activity is >3,500 pmol/min/μg, as measured under the described conditions.

Formulation, Preparation and Storage

5906-ZN
Formulation Supplied as a 0.2 μm filtered solution in Tris and NaCl.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: Carboxypeptidase A4/CPA4

Carboxypeptidase A4, also known as CPA4, is a secreted, zinc-dependent metallocarboxypeptidase that removes the C-terminal amino acid from peptides having a free C-terminal carboxyl group. CPA4 can hydrolyze both amide and ester bonds and has a preference for cleavage at the amino side of hydrophobic residues. CPA4 is inhibited by latexin, an endogenous inhibitor of carboxypeptidases (1). The deduced amino acid sequence of human CPA4 consists of a signal peptide (residues 1‑16), a pro region (17‑113), and a mature chain (residues 114‑421).  The CPA4 gene has been associated with prostate cancer aggressiveness and is involved in the histone hyperacetylation signaling pathway (2, 3).  Recent studies show that coding variation in the CPA4 gene is linked to high risk prostate cancer among younger patients (4).

References

  1. Pallares, I. et al. (2005) Proc. Natl. Acad. Sci. USA 102:3978.
  2. Kayashima, T. et al. (2003) Hum. Genet. 3:220.
  3. Huang, H. et al. (1999) Cancer Research. 59:2981.
  4. Ross, P.L. et al. (2009) BMC Cancer. 9:69.

Alternate Names

CPA4

Entrez Gene IDs

51200 (Human); 71791 (Mouse)

Gene Symbol

CPA4

UniProt

Q9UI42

Additional Carboxypeptidase A4/CPA4 Products

Product Documents for Recombinant Human Carboxypeptidase A4/CPA4 Protein, CF

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Product Specific Notices for Recombinant Human Carboxypeptidase A4/CPA4 Protein, CF

For research use only

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