Recombinant Human Carboxypeptidase M Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 7457-ZN

R&D Systems, part of Bio-Techne
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Key Product Details

Source

CHO

Accession #

P14384

Conjugate

Unconjugated

Applications

Enzyme Activity

View all Carboxypeptidase M Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human Carboxypeptidase M protein
Leu18-Ser423, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 μg per lane.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Leu18

Predicted Molecular Mass

47 kDa

SDS-PAGE

50-56 kDa, reducing conditions

Activity

Measured by its ability to release L-arginine from Benzoyl-Ala-Arg, with detection of the arginine amino group by o-phthaldialdehyde.
The specific activity is >40,000 pmol/min/μg, as measured under the described conditions.

Formulation, Preparation and Storage

7457-ZN
Formulation Supplied as a 0.2 μm filtered solution in MES and NaCl.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: Carboxypeptidase M

Carboxypeptidase M (CPM) is a zinc metallopeptidase specific for the removal of arginine and lysine residues from peptides. CPM is bound to the plasma membrane through a glycosylphosphatidylinositol anchor (1). The enzyme is thought to regulate the actions of some peptide hormones at the cell surface through their degradation (2). CPM is a biomarker for well-differentiated liposarcomas (3) and is also a marker for the maturation of monocytes to macrophages (4). CPM binds to the kinin B1 receptor on the cell surface, forming a complex that potentiates the signaling ability of the kinin B1 receptor (5).  Recombinant human CPM was expressed as a C-terminally truncated protein to prevent the formation of the glycosylphosphatidylinositol anchor, resulting in the secretion of the protein.

References

  1. Tan F. et al. (2003) Biochem. J. 370:567.

  2. Reverter D. et al. (2004) J. Mol. Biol. 338:257.

  3. Erickson-Johnson M.R. et al. (2009) Mod. Pathol. 22:1541.

  4. Rehli M. et al. (2000) Adv. Exp. Med. Biol. 477:205.

  5. Zhang X. et al. (2011) J. Biol. Chem. 286:18547.

Alternate Names

CPM

Entrez Gene IDs

1368 (Human); 70574 (Mouse); 314855 (Rat)

Gene Symbol

CPM

UniProt

P14384 (Human)

Additional Carboxypeptidase M Products

Product Documents for Recombinant Human Carboxypeptidase M Protein, CF

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Human Carboxypeptidase M Protein, CF

Coomassie is a registered trademark of Imperial Chemical Industries Ltd. Triton is a registered trademark of Union Carbide Corp.

For research use only

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