Recombinant Human CART Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 10974-CO

R&D Systems, part of Bio-Techne
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10974-CO-050
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Key Product Details

Source

E. coli

Accession #

Q16568.1

Conjugate

Unconjugated

Applications

Bioactivity

View all CART Proteins and Enzymes »

Product Specifications

Source

E. coli-derived human CART protein
Ala37-Leu116

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ala37

Predicted Molecular Mass

9 kDa

SDS-PAGE

8-10 kDa, reducing conditions.

Activity

Measured by its ability to enhance neurite outgrowth of E16-E18 rat embryonic cortical neurons.
Recombinant Human CART, immobilized at 5.0 μg/mL on a 96 well plate, is able to significantly enhance neurite outgrowth.

Scientific Data Images for Recombinant Human CART Protein, CF

Recombinant Human CART Protein SDS-PAGE.

2 μg/lane of Recombinant Human CART Protein (Catalog # 10974-CO) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 8-10 kDa under reducing conditions.

Formulation, Preparation and Storage

10974-CO
Formulation Lyophilized from a 0.2 μm filtered solution in HCl.
Reconstitution Reconstitute at 500 μg/mL in 4 mM HCl.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CART

Cocaine and amphetamine regulated transcript, also known as CART, is a neuropeptide protein encoded in human by the CARPT gene (1, 2) and is expressed in brain regions associated with reward such as the nucleus accumbens, also expressed in retinal cells, in pituitary glands, adrenal medulla and pancreas (3, 4). CART peptides can function as neurotransmitters and hormones, promote neuronal development, and are involved in regulating many processes, including food intake, maintenance of body weight, reward and addiction, stress response, psychostimulant effects, and endocrine function (5-7). CART production is up-regulated by CREB (8) a protein thought to be involved with the development of drug addiction, thus CART may be an important therapeutic target in the treatment of stimulant abuse (9). More recently, studies show that CART also has a wide variety of functions including bone remodeling, depression, anxiety, learning and memory as well as physiological processes such osmoregulation and blood pressure (10, 11). CART treatment promotes neuronal development and survival in vitro and in vivo (12-14).

References

  1. Douglas, J. and S. Daoud (1996) Gene 169:241.
  2. Kristensen, P. et al. (1998) Nature 303:72.
  3. Keller, P.A. et al. (2006) Peptides 27:1328.
  4. Jensen, P.B. et al. (1999) FEBS Lett 447:139.
  5. Rogge, G. et al. (2008) Nat. Rev. Neurosci. 9:747.
  6. ChengPeng, Y. et al. (2017) Front. Cell. Neurosci 11:244.
  7. Lau, J. and H. Herzog (2014) Front. Neurosci. 8:313.
  8. Rogge, G.A. et al. (2009) Brain Res. 1251:42.
  9. Fagergren, P. and Y. Hurd (2007) Physiol. Behav. 92:218.
  10. Ahmadian-Moghadam, H. et al. (2018) Peptides 110:56.
  11. Zhi, Y.O. and P. Gavan (2020) Brain Res. 1740:146852.
  12. Wu, B. et al. (2006) Biochem. Biophys. Res. 347:656.
  13. Jin, J.L. et al. (2014) Nature 5:10224.
  14. Sha, D.J. et al. (2013) Neuroreport. 24:698.

Long Name

Cocaine- and Amphetamine-regulated Transcript Protein

Alternate Names

CARTPT

Entrez Gene IDs

9607 (Human)

Gene Symbol

CARTPT

UniProt

Q16568.1

Additional CART Products

Product Documents for Recombinant Human CART Protein, CF

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Product Specific Notices for Recombinant Human CART Protein, CF

For research use only

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