Recombinant Human Caspr2 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 8207-CR

R&D Systems, part of Bio-Techne
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8207-CR-050
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Key Product Details

Source

NS0

Accession #

Q9UHC6

Conjugate

Unconjugated

Applications

Bioactivity

View all Caspr2 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human Caspr2 protein
Pro29-Ala1262, with a C-terminal 6-His tag

Purity

>90%, by SDS-PAGE with silver staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Pro29

Predicted Molecular Mass

138 kDa

SDS-PAGE

130-150 kDa, reducing conditions

Activity

Bioassay data are not available.

Formulation, Preparation and Storage

8207-CR
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 250 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
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Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Caspr2

Contact-associated Protein-like 2 (Caspr2) is a type I transmembrane member of the neurexin family of adhesion molecules (1). Human Caspr2 is the vertebrate homolog to Drosophila Neurexin IV, and the human and mouse Caspr2 orthologs share 94% amino acid sequence identity. Human Caspr2 contains two EGF-like domains, one F5/8 type C domain, one fibrinogen C-terminal domain, and four laminin G-like domains. It is highly expressed in neuronal tissue where it is primarily localized to the juxtaparanodal region of the axonal membrane (1, 2). Caspr2 acts in conjunction with 4.1B and Tag-1 as a scaffold that clusters Kv1 potassium channels at the juxtaparanodal region and is critical for axo-glial contacts (1-6). Caspr2 interacts with carboxypeptidase E in Golgi bodies during intracellular trafficking to the cell membrane (7). Caspr2 is also required for dendrite arborization and the normal development of neural networks (8). Mutations in Caspr2 are associated with predisposition to autism spectrum disorders, epilepsy, attention-deficit hyperactive disorder, and schizophrenia (8-11). The presence of autoantibodies against Caspr2 is associated with encephalitis, epilepsy, dysarthria, and paroxysmal kinesigenic dystonia (12-14). R&D Systems in-house testing indicates that Caspr2 can enhance bovine corneal endothelial cell adhesion.

References

  1. Poliak, S. et al. (1999) Neuron 24:1037.
  2. Poliak, S. et al. (2001) J. Neurosci. 21:7568.
  3. Horresh, I. et al. (2010) J. Neurosci. 30:2480.
  4. Traka, M. et al. (2003) J. Cell Biol. 162:1161.
  5. Denisenko-Nehrbass, N. et al. (2003) Eur. J. Neurosci. 17:411.
  6. Poliak, S. et al. (2003) J. Cell Biol. 162:1149.
  7. Oiso, S. et al. (2009) J. Neurochem. 109:158.
  8. Anderson, G.R. et al. (2012) Proc. Natl. Acad. Sci. USA 109:18120.
  9. Penagarikano, O. and D.H. Geschwind (2012) Trends Mol. Med. 18:156.
  10. Friedman, J.I. et al. (2008) Mol. Psychiatry 13:261.
  11. Elia, J. et al. (2010) Mol. Psychiatry 15:637.
  12. Lancaster, E. et al. (2011) Ann. Neurol. 69:303.
  13. Balint, B. et al. (2013) J. Neurol. Sci. 327:73.
  14. Krogias, C. et al. (2013) JAMA Neurol. 70:1056.

Long Name

Contactin Associated Protein-like 2

Alternate Names

CDFE, CNTNAP2, NRXN4

Entrez Gene IDs

26047 (Human); 66797 (Mouse)

Gene Symbol

CNTNAP2

UniProt

Q9UHC6

Additional Caspr2 Products

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Product Specific Notices for Recombinant Human Caspr2 Protein, CF

For research use only

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