Recombinant Human Cathepsin E Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 1294-AS
Key Product Details
Source
NS0
Accession #
Structure / Form
Pro and mature forms
Conjugate
Unconjugated
Applications
Enzyme Activity
Product Specifications
Source
Mouse myeloma cell line, NS0-derived human Cathepsin E protein
Gln18-Pro396, with a C-terminal 10-His tag
Gln18-Pro396, with a C-terminal 10-His tag
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Ile54 & Gln18 (predicted)
Predicted Molecular Mass
42 kDa
SDS-PAGE
48 kDa, reducing conditions
Activity
Measured by its ability to cleave the fluorogenic peptide substrate, Mca-PLGL-Dpa-AR-NH2 (Catalog # ES001).
The specific activity is >1,500 pmol/min/µg, as measured under the described conditions.
The specific activity is >1,500 pmol/min/µg, as measured under the described conditions.
Formulation, Preparation and Storage
1294-AS
| Formulation | Lyophilized from a 0.2 μm filtered solution in MES and NaCl. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile 25 mM MES, 150 mM NaCl, pH 6.5. |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: Cathepsin E
Cathepsin E is an intracellular aspartic protease of the pepsin family (1). Unlike Cathepsin D, another member of the same family and a lysosomal protease with relatively ubiquitous distribution, Cathepsin E is not a lysosomal enzyme and has a limited cell and tissue distribution. However, both Cathepsin D and E play an important role in the degradation of proteins, the generation of bioactive proteins, and antigen processing (2). Both enzymes are efficient in cleaving Swedish mutant of amyloid precursor protein (APP) at the beta site but show almost no reactivity with wild-type APP (3). Human Cathepsin E is synthesized as a precursor protein, consisting of a signal peptide (residues 1‑17), a propeptide (residues 18‑53), and a mature chain (residues 54‑396) (4).
References
- Kay, J. and P.J. Tatnell (2004) in Handbook of Proteolytic Enzymes (Barrett, A.J. et al. eds.), p. 33, Academic Press, San Diego.
- Tsukuba, T. et al. (2000) Mol. Cells 10:601.
- Gruninger-Leitch, F. et al. (2000) Nat. Biotechnol. 18:66.
- Azuma, T. et al. (1989) J. Biol. Chem. 264:16748.
Alternate Names
CTSE
Gene Symbol
CTSE
UniProt
Additional Cathepsin E Products
Product Documents for Recombinant Human Cathepsin E Protein, CF
Product Specific Notices for Recombinant Human Cathepsin E Protein, CF
For research use only
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