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Recombinant Human Cathepsin H Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 7516-CY

R&D Systems, part of Bio-Techne
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7516-CY-010

Key Product Details

Source

CHO

Accession #

Conjugate

Unconjugated

Applications

Enzyme Activity

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human Cathepsin H protein
Ala23-Val335, with a C-terminal 10-His tag.

Purity

>80%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ala23

Predicted Molecular Mass

37 kDa

SDS-PAGE

38-43 kDa, reducing conditions

Activity

Measured by its ability to cleave the fluorogenic peptide substrate, Arg-7-amido-4-methylcoumarin (R-AMC).
The specific activity is >750 pmol/min/μg, as measured under the described conditions.

Formulation, Preparation and Storage

7516-CY
Formulation Supplied as a 0.2 μm filtered solution in Tris and NaCl.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.

Background: Cathepsin H

Cathepsin H is a lysosomal cysteine protease of the papain family (1). It is synthesized as a precursor protein that is proteolytically processed to a mature form consisting of a light chain, a heavy chain, and a mini-chain (2). Cathepsin H is the only known mono-aminopeptidase in the papain family (3). Cathepsin H is primarily an aminopeptidase, but also functions as an endopeptidase (4). Cathepsin H is potently inhibited by cystatins A, B, and C and by ovocystatin. Cathepsin H expression is altered in disease states such as prostate and colorectal cancers (5).

References

  1. Kirshke, H. (2004) in Handbook of Proteolytic Enzymes (ed. Barrett et al.) p. 1089, Academic Press, San Diego.
  2. Fuchs, R. et al. (1988) Biol. Chem. Hoppe-Seyler 369:469.
  3. Guncar, G. et al. (1998) Structure 310:113.
  4. Kirschke, H. et al. (1977) Acta Biol. Med. Ger. 6:51.
  5. del Re, E.C. et al. (2000) Br. J. Cancer 82:1317.

Alternate Names

CTSH

Entrez Gene IDs

1512 (Human); 13036 (Mouse)

Gene Symbol

CTSH

UniProt

Additional Cathepsin H Products

Product Documents for Recombinant Human Cathepsin H Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human Cathepsin H Protein, CF

For research use only

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