Recombinant Human Cathepsin S Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 1183-CY
Key Product Details
Source
NS0
Accession #
Structure / Form
Pro and mature forms
Conjugate
Unconjugated
Applications
Enzyme Activity
Product Specifications
Source
Mouse myeloma cell line, NS0-derived human Cathepsin S protein
Gln17-Ile331 (pro) & Ser109-Ile331 (mature), both with a C-terminal 10-His tag
Gln17-Ile331 (pro) & Ser109-Ile331 (mature), both with a C-terminal 10-His tag
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Gln17 predicted & Ser109
Predicted Molecular Mass
39 kDa (Pro) and 28 kDa (mature)
SDS-PAGE
37-41 kDa and 26-27 kDa, reducing conditions
Activity
Measured by its ability to cleave the fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK(Dnp)-NH2 (Catalog # ES002).
The specific activity is >300 pmol/min/µg, as measured under the described conditions.
The specific activity is >300 pmol/min/µg, as measured under the described conditions.
Reviewed Applications
Read 1 review rated 5 using 1183-CY in the following applications:
Formulation, Preparation and Storage
1183-CY
| Formulation | Supplied as a 0.2 μm filtered solution in MES, NaCl and Glycerol. |
| Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: Cathepsin S
Cathepsin S is a lysosomal cysteine protease of the papain family (1). It plays a major role in the processing of the MHC class II‑associated invariant chain (2). It has been implicated in the pathogenesis of several diseases such as Alzheimer’s disease and degenerative disorders associated with the cells of the mononuclear phagocytic system (1). Human Cathepsin S is synthesized as a preproenzyme of 331 amino acid residues consisting a signal peptide (residues 1‑16), a pro region (residues 17‑114), and the mature enzyme (residues 115‑331) (3‑5). Cathepsin S is less abundant in tissues than Cathepsins B, L and H. The highest levels have been found in lymph nodes, spleen, macrophages and other phagocytic cells.
References
- Kirschke, H. (2004) in Handbook of Proteolytic Enzymes (ed. Barrett, A.J. et al.) pp. 1104 - 1107, Academic Press, San Diego.
- Turk, V. et al. (2001) EMBO J. 20:4629.
- Shi, G.P. et al. (1992) J. Biol. Chem. 267:7258.
- Shi, G.P. et al. (1994) J. Biol. Chem. 269:11530.
- Wiederanders, B. et al. (1992) J. Biol. Chem. 267:13708.
Alternate Names
CTSS
Entrez Gene IDs
1520 (Human)
Gene Symbol
CTSS
UniProt
Additional Cathepsin S Products
Product Documents for Recombinant Human Cathepsin S Protein, CF
Product Specific Notices for Recombinant Human Cathepsin S Protein, CF
For research use only
Loading...
Loading...
Loading...