Recombinant Human CCL18/PARC Protein
R&D Systems, part of Bio-Techne | Catalog # 394-PA
Key Product Details
Product Specifications
Source
E. coli-derived human CCL18/PARC protein
Ala21-Ala89
Ala21-Ala89
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Ala21
Predicted Molecular Mass
7.8 kDa
Activity
Measured by its ability to inhibit Eotaxin-induced chemotaxis of BaF3 mouse pro-B cells transfected with human CCR3. Nibbs, RJB. et al. (2000) J. Immunol. 164:1488.
The ED50 for this effect is 1-5 µg/mL.
The ED50 for this effect is 1-5 µg/mL.
Reviewed Applications
Read 2 reviews rated 4.5 using 394-PA in the following applications:
Formulation, Preparation and Storage
Carrier Free
What does CF mean?CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
What formulation is right for me?In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
Carrier: 394-PA
| Formulation | Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage |
|
Carrier Free: 394-PA/CF
| Formulation | Lyophilized from a 0.2 μm filtered solution in Acetonitrile with Trehalose. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage |
|
Background: CCL18/PARC
CCL18, also known as alternative macrophage activation-associated CC chemokine (AMAC)-1, macrophage inflammatory protein (MIP)-4, and dendritic cell chemokine (DC-CK1), is a novel CC chemokine that is highly homologous to MIP-1 alpha (61% amino acid (aa) sequence identity). CCL18 cDNA encodes an 89 aa residue precursor protein with a 20 aa putative signal peptide that is cleaved to generate a 69 aa residue mature protein which lacks potential glycosylation sites. In vitro, CCL18 mRNA expression is induced in alternatively activated macrophages by Th2 cytokines such as IL-4, IL-10 and IL-13, and inhibited by IFN‑ gamma. CCL18 mRNA is also expressed by GM‑CSF/IL‑4‑induced monocyte-derived dendritic cells. In vivo, CCL18 is highly expressed in lung and placenta but is not expressed in epidermal Langerhans cells. Recombinant CCL18 has been shown to chemoattract naive T cells, but not monocytes or neutrophils.
References
- Adema, G. et al. (1997) Nature 387:713.
- Kodelja, V. et al. (1998) J. Immunol. 160:141.
Alternate Names
AMAC-1, DC-CK1, MIP-4, PARC
Entrez Gene IDs
6362 (Human)
Gene Symbol
CCL18
UniProt
Additional CCL18/PARC Products
Product Documents for Recombinant Human CCL18/PARC Protein
Product Specific Notices for Recombinant Human CCL18/PARC Protein
For research use only
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