Recombinant Human CCL20/MIP-3 alpha Protein
R&D Systems, part of Bio-Techne | Catalog # 360-MP
Key Product Details
Product Specifications
Source
E. coli-derived human CCL20/MIP-3 alpha protein
Ala27-Met96 (Asn95Asp)
Ala27-Met96 (Asn95Asp)
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Ala27
Predicted Molecular Mass
8 kDa
Activity
Measured by its ability to chemoattract BaF3 mouse pro-B cells transfected with human CCR6.
The ED50 for this effect is 0.5-2 ng/mL.
The ED50 for this effect is 0.5-2 ng/mL.
Reviewed Applications
Read 2 reviews rated 5 using 360-MP in the following applications:
Scientific Data Images for Recombinant Human CCL20/MIP-3 alpha Protein
Recombinant Human CCL20/MIP-3 alpha Protein Bioactivity
Recombinant Human CCL20/MIP-3 alpha (Catalog # 360-MP) chemoattracts the BaF3 mouse pro-B cells transfected with human CCR6. The ED50 for this effect is 0.5‑2 ng/mL.
Recombinant Human CCL20/MIP-3 alpha Protein SDS-PAGE
1 μg/lane of Recombinant Human CCL20/MIP-3 alpha was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 8 kDa.Formulation, Preparation and Storage
Carrier Free
What does CF mean?CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
What formulation is right for me?In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
Carrier: 360-MP
| Formulation | Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein. |
| Reconstitution | Reconstitute at 25 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Carrier Free: 360-MP/CF
| Formulation | Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: CCL20/MIP-3 alpha
CCL20, also known as LARC (Liver and Activation-regulated Chemokine) and as Exodus, is one of many novel beta chemokines identified through bioinformatics. CCL20 cDNA encodes a 96 amino acid (aa) residue precursor protein with a 26 aa residue signal peptide that is predicted to be cleaved to form the 70 aa residue mature secreted protein. CCL20 is distantly related to other beta chemokines (20‑28% aa sequence identity) and the gene for CCL20 has been mapped to chromosome 2 rather than 17.
CCL20 has been shown to be expressed predominantly in lymph nodes, appendix, PBL, fetal liver, fetal lung and several cell lines. The expression of CCL20 is strongly up-regulated by inflammatory signals and down-regulated by the anti-inflammatory cytokine IL-10. Synthetic or recombinant CCL20 has been shown to be chemotactic for lymphocytes and to inhibit proliferation of myeloid progenitors in colony formation assays. CCL20 has now been shown to be a unique functional ligand for CCR-6 (previously referred to as GPR-CY4, CKR-L3, or STRL22 orphan receptor), a chemokine receptor that is selectively and highly expressed in human dendritic cells derived from CD34+ cord blood precursors.
References
- Baba, M. et al. (1997) J. Biol. Chem. 272:14893.
- Hromas, R. et al. (1997) Blood 89:3315.
- Greaves, D.R. et al. (1997) J. Exp. Med. 186:837.
Alternate Names
exodus-1, LARC, MIP-3 alpha, MIP3 alpha
Gene Symbol
CCL20
UniProt
Additional CCL20/MIP-3 alpha Products
Product Documents for Recombinant Human CCL20/MIP-3 alpha Protein
Product Specific Notices for Recombinant Human CCL20/MIP-3 alpha Protein
For research use only
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