Recombinant Human CCL7/MCP-3 Protein
R&D Systems, part of Bio-Techne | Catalog # 282-P3
Key Product Details
Product Specifications
Source
E. coli-derived human CCL7/MCP-3/MARC protein
Gln34-Leu109
Gln34-Leu109
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Gln34
Predicted Molecular Mass
9 kDa
Activity
Measured by its ability to chemoattract 2-day cultured human monocytes. Matsushima, K. et al. (1989) J. Exp. Med. 169:1485.
The ED50 for this effect is 0.02-0.08 µg/mL.
Measured by its ability to chemoattract BaF3 mouse pro-B cells transfected with human CCR2A.
The ED50 for this effect is 0.1-0.5 µg/mL.
The ED50 for this effect is 0.02-0.08 µg/mL.
Measured by its ability to chemoattract BaF3 mouse pro-B cells transfected with human CCR2A.
The ED50 for this effect is 0.1-0.5 µg/mL.
Reviewed Applications
Read 2 reviews rated 5 using 282-P3 in the following applications:
Formulation, Preparation and Storage
Carrier Free
What does CF mean?CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
What formulation is right for me?In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
Carrier: 282-P3
| Formulation | Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Carrier Free: 282-P3/CF
| Formulation | Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: CCL7/MCP-3/MARC
MCP-2 and CCL7 are two monocyte chemotactic proteins produced by human MG-63 osteosarcoma cells. Both MCP-2 and CCL7 are members of the C-C family of chemokines and share 62% and 71% amino acid sequence identity, respectively, with MCP-1. CCL7 also shares 58% amino acid identity with MCP-2.
CCL7 cDNA encodes a 99 amino acid residue precursor protein from which the N-terminal 23 amino acid residues are cleaved to generate the 76 amino acid residue mature CCL7. Mature CCL7 contains a potential N-linked and several possible O-linked glycosylation sites.
Similarly to other C-C chemokines, all three MCP proteins are monocyte chemoattractants. In addition, the three MCPs can chemoattract activated NK cells as well as CD4+ and CD8+ T lymphocytes. All three cytokines have also been shown to attract eosinophils and induce histamine secretion from basophils.
Alternate Names
MARC, MCP-3
Gene Symbol
CCL7
UniProt
Additional CCL7/MCP-3/MARC Products
Product Documents for Recombinant Human CCL7/MCP-3 Protein
Product Specific Notices for Recombinant Human CCL7/MCP-3 Protein
For research use only
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