Recombinant Human CD19 Fc Chimera Avi-tag Protein, CF

R&D Systems, part of Bio-Techne | Catalog # AVI9269

Analyzed by SEC-MALS. Biotinylated.
R&D Systems, part of Bio-Techne
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AVI9269-050
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Key Product Details

Learn more about Avi-tag Biotinylated Proteins

Source

CHO

Accession #

P15391

Structure / Form

Disulfide-linked homodimer, biotinylated via Avi-tag

Conjugate

Biotin

Applications

Bioactivity

View all CD19 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human CD19 protein
Human CD19
(Glu21-Lys291)
Accession # P15391		 IEGRMD Human IgG1
(Pro100-Lys330)		 Avi-tag
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Glu21

Predicted Molecular Mass

59 kDa

SDS-PAGE

75-95 kDa, under reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Human CD19 Antibody (FMC63) (Novus Biologicals, Catalog # NBP2-52716) is immobilized at 0.5 µg/mL (100 µL/well), it binds to Biotinylated Recombinant Human CD19 Fc Chimera Avi-tag (Catalog # AVI9269) with an ED50 of 7.5-45 ng/mL.

Scientific Data Images for Recombinant Human CD19 Fc Chimera Avi-tag Protein, CF

Biotinylated Recombinant Human CD19 Fc Chimera Avi-tag Protein SEC-MALS.

Recombinant Human CD19 Fc Chimera Avi-tag (Catalog # AVI9269) has a molecular weight (MW) of 133.5 kDa as analyzed by SEC-MALS, suggesting that this protein is a homodimer. MW may differ from predicted MW due to post-translational modifications (PTMs) present (i.e. Glycosylation).
Recombinant Human CD19 Fc Chimera Avi-tag Protein Binding Activity

Recombinant Human CD19 Fc Chimera Avi-tag Protein Binding Activity

When Human CD19 antibody (FMC63) (Novus Biologicals Catalog # NBP2-52716) is immobilized at 0.5 μg/mL, 100 μL/well, Recombinant Human CD19 Fc Chimera Avi-tag (Catalog # AVI9269) binds with an ED50 of 7.5-45 ng/mL.
Recombinant Human CD19 Fc Chimera Avi-tag Protein SDS-PAGE

Recombinant Human CD19 Fc Chimera Avi-tag Protein SDS-PAGE

2 μg/lane of Recombinant Human CD19 Fc Chimera Avi-tag (Catalog # AVI9269) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 75-95 kDa and 150-190 kDa, respectively.

Formulation, Preparation and Storage

AVI9269
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD19

CD19, also known as B4, is a transmembrane glycoprotein of the immunoglobulin superfamily that plays a central role in B cell activation and humoral immune responses (1, 2). CD19 consists of an extracellular domain (ECD) with two C2-type Ig-like domains, a transmembrane segment, and a cytoplasmic domain with nine tyrosine residues, 3 of which are critical for function (1, 2). Within the mature ECD, human CD19 shares 57% amino acid sequence identity with mouse and rat CD19. CD19 is expressed throughout B cell development from pre‑B cells through mature B cells, and it is commonly used as a B cell lineage marker (1, 2). It is required for the responsiveness of mature B cell to antigen stimulation, germinal center development, and antibody affinity maturation (1, 2). CD19 associates with the B cell antigen receptor (BCR), CD81, CD38, CD21, CD22, and IFITM1/CD225/Leu-13 (1, 3). These associations enable CD19 to amplify B cell signaling and reduce the threshold for antigen stimulation through the BCR (1, 3). CD19 polymorphisms and up-regulation can lead to the development of autoimmunity by promoting autoantibody production (2). CD19 has emerged as promising therapeutic target for hematologic cancers and solid tumors, such as leukemias and lymphomas (4, 5). Immunotherapy using a chimeric antigen receptor (CAR) targeting CD19 has emerged as promising therapeutic target for hematologic cancers and solid tumors, such as leukemias and lymphomas (4, 5). The first CD19 CAR T cell therapies have been granted FDA approval for the treatment of B cell malignancies with several more in clinical trials (6). Our Avi-tag Biotinylated TGF-beta 1 features biotinylation at a single site contained within the Avi-tag, a unique 15 amino acid peptide. Protein orientation will be uniform when bound to streptavidin-coated surface due to the precise control of biotinylation and the rest of the protein is unchanged so there is no interference in the protein's bioactivity.

References

  1. Wang, K. et al. (2012) Exp. Hematol. Oncol. 1:36.
  2. Del Nargo, C.J. et al. (2005) Immunol. Res. 31:229.
  3. Yu, F. et al. (2010) J. Neurooncol. 103:187.
  4. Kochenderfer, J. et al. (2015) J Clin. Oncol. 33:540.
  5. Lee, D. et al. (2015) Lancet. 385:517.
  6. Ahmad, A. et al. (2020) Int. J. Mol. Sci. 21:3906.

Alternate Names

CD19, CVID3, Leu-12

Entrez Gene IDs

930 (Human); 12478 (Mouse); 365367 (Rat); 397669 (Porcine); 607898 (Canine); 102145514 (Cynomolgus Monkey); 100147468 (Equine)

Gene Symbol

CD19

UniProt

P15391 (Human)

Additional CD19 Products

Product Documents for Recombinant Human CD19 Fc Chimera Avi-tag Protein, CF

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Human CD19 Fc Chimera Avi-tag Protein, CF

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