Recombinant Human CD19 His-tag Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 11095-CD

R&D Systems, part of Bio-Techne
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11095-CD-050
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Key Product Details

Source

CHO

Accession #

P15391.6

Conjugate

Unconjugated

Applications

Bioactivity

View all CD19 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human CD19 protein
Human CD19
(Glu21-Lys291)
Accession # P15391.6		 6-His tag IEGR
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Glu21

Predicted Molecular Mass

31 kDa

SDS-PAGE

50-65 kDa, under reducing conditions.

Activity

Measured by its binding ability in a functional ELISA.
When Human CD19 Antibody (FMC63) (Novus Biologicals, Catalog # NBP2-52716) is immobilized at 2.00 µg/mL (100 µL/well), Recombinant Human CD19 binds with an ED50 of 15.0-150 ng/mL.

Scientific Data Images for Recombinant Human CD19 His-tag Protein, CF

Recombinant Human CD19 His-tag Protein Binding Activity.

When Human CD19 Antibody (FMC63) (Novus Biologicals, Catalog # NBP2-52716) is immobilized at 2.00 µg/mL (100 µL/well), Recombinant Human CD19 His-tag Protein (Catalog # 11095-CD) binds with an ED50 of 15.0-50 ng/mL.

Recombinant Human CD19 His-tag Protein SDS-PAGE.

2 μg/lane of Recombinant Human CD19 His-tag Protein (Catalog # 11095-CD) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 50-65 kDa.

Formulation, Preparation and Storage

11095-CD
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD19

CD19, also known as B4, is a transmembrane glycoprotein of the immunoglobulin superfamily that plays a central role in B cell activation and humoral immune responses (1, 2). CD19 consists of an extracellular domain (ECD) with two C2-type Ig-like domains, a transmembrane segment, and a cytoplasmic domain with nine tyrosine residues, 3 of which are critical for function (1, 2). Within the mature ECD, human CD19 shares 57% amino acid sequence identity with mouse and rat CD19. CD19 is expressed throughout B cell development from pre‑B cells through mature B cells, and it is commonly used as a B cell lineage marker (1, 2). It is required for the responsiveness of mature B cell to antigen stimulation, germinal center development, and antibody affinity maturation (1, 2). CD19 associates with the B cell antigen receptor (BCR), CD81, CD38, CD21, CD22, and IFITM1/CD225/Leu-13 (1, 3). These associations enable CD19 to amplify B cell signaling and reduce the threshold for antigen stimulation through the BCR (1, 3). CD19 polymorphisms and up-regulation can lead to the development of autoimmunity by promoting autoantibody production (2). CD19 has emerged as promising therapeutic target for hematologic cancers and solid tumors, such as leukemias and lymphomas (4, 5). Immunotherapy using a chimeric antigen receptor (CAR) targeting CD19 has emerged as promising therapeutic target for hematologic cancers and solid tumors, such as leukemias and lymphomas (4, 5). The first CD19 CAR T cell therapies have been granted FDA approval for the treatment of B cell malignancies with several more in clinical trials (6).

References

  1. Wang, K. et al. (2012) Exp. Hematol. Oncol. 1:36.
  2. Del Nargo, C.J. et al. (2005) Immunol Res. 31:229.
  3. Yu, F. et al. (2010) J Neurooncol. 103:187.
  4. Kochenderfer, J. et al. (2015) J. Clin. Oncol. 33:540.
  5. Lee, D. et al. (2015) Lancet. 385:517.
  6. Ahmad, A. et al. (2020) Int. J. Mol. Sci. 21:3906.

Alternate Names

CD19, CVID3, Leu-12

Entrez Gene IDs

930 (Human); 12478 (Mouse); 365367 (Rat); 397669 (Porcine); 607898 (Canine); 102145514 (Cynomolgus Monkey); 100147468 (Equine)

Gene Symbol

CD19

UniProt

P15391.6

Additional CD19 Products

Product Documents for Recombinant Human CD19 His-tag Protein, CF

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Product Specific Notices for Recombinant Human CD19 His-tag Protein, CF

For research use only

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