Recombinant Human CD200R1 His-tag Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 10053-CD

R&D Systems, part of Bio-Techne
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10053-CD-050
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Key Product Details

Source

HEK293

Accession #

Q8TD46

Conjugate

Unconjugated

Applications

Bioactivity

View all CD200R1 Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human CD200R1 protein
Ala27-Leu266, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ala27

Predicted Molecular Mass

28 kDa

SDS-PAGE

43-60 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinan Human CD200 R1 is immobilized at 2 µg/mL (100 µL/well), the concentration of Recombinant Human CD200 Fc Chimera (Catalog # 2724-CD) that produces 50% of the optimal binding response is 2.5-15 ng/mL.

Reviewed Applications

Read 1 review rated 5 using 10053-CD in the following applications:

Scientific Data Images for Recombinant Human CD200R1 His-tag Protein, CF

Recombinant Human CD200R1 His-tag Protein Binding Activity

Recombinant Human CD200R1 His-tag Protein Binding Activity

When Recombinant Human CD200 R1 (Catalog # 10053-CD) is coated at 2 µg/mL, 100 µL/well, Recombinant Human CD200 Fc Chimera (Catalog # 2724-CD) binds with an ED50 of 2.5-15 ng/mL.
Recombinant Human CD200R1 His-tag Protein SDS-PAGE

Recombinant Human CD200R1 His-tag Protein SDS-PAGE

2 μg/lane of Recombinant Human CD200 R1 was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 43-60 kDa.

Formulation, Preparation and Storage

10053-CD
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD200R1

CD200 R1, also known as OX-2 receptor, is a 90 kDa transmembrane protein in the immunoglobulin superfamily and is important in the regulation of myeloid cell activity (1-3). The human CD200 R1 cDNA encodes a 325 amino acid (aa) precursor that includes a 28 aa signal sequence, a 215 aa extracellular domain (ECD), a 21 aa transmembrane segment, and a 61 aa cytoplasmic domain. The ECD is composed of one Ig-like V-type domain and one Ig-like C2-type domain (4). Within the ECD, human CD200 R1 shares 56% aa sequence identity with both mouse and rat CD200 R1. Alternate splicing of the human CD200 R1 mRNA generates four isoforms, two of which are truncated in the Ig-C2 domain and are likely secreted (4). In human, a separate CD200 RL gene encodes a protein that shares 81% ECD aa identity with CD200 R1 (5). In mouse, at least four genes for CD200 R1-like molecules have been described (5-7). CD200 R1 expression is restricted primarily to mast cells, basophils, macrophages, and dendritic cells (8-10), while its ligand, CD200, is widely distributed (11). Disruption of this receptor-ligand system by knockout of the CD200 gene in mice leads to increased macrophage number and activation and predisposition to autoimmune disorders (12). Association of CD200 with CD200 R1 takes place between their respective N-terminal Ig-like domains (13). The capacity of CD200 R1-like molecules to interact with CD200 is controversial (6, 14). CD200 R1 propagates inhibitory signals despite lacking a cytoplasmic ITIM (immunoreceptor tyrosine-based inhibitory motif) (9, 10, 15, 16). CD200 R1-like molecules, in contrast, are potentially activating receptors by means of their association with DAP12 (5, 7). CD200R1 signaling inhibits the expression of proinflammatory molecules including TNFs, IFNs, and inducible nitric oxide synthase in response to selected stimuli, which implicate that CD200/CD200R1 inhibitory signaling pathway plays a prominent role in limiting inflammation in a wide range of inflammatory diseases (17). Furthermore, the CD200/CD200R inhibitory signaling constitutes one of the most suitable endogenous immunoregulatory molecule candidate to restore the immune suppressive status of the CNS altered in chronic neuroinflammatory situations (18).

References

  1. Rosenblum, M.D. et al. (2006) J. Dermatol. Sci. 41:165.
  2. Gorczynski, R.M. (2005) Curr. Opin. Invest. Drugs 6:483.
  3. Barclay, A.N. et al. (2002) Trends Immunol. 23:285.
  4. Vieites J.M. et al. (2003) Gene. Jun. 5; 311:99.
  5. Wright, G.J. et al. (2003) J. Immunol. 171:3034.
  6. Hatherley, D. et al. (2005) J. Immunol. 175:2469.
  7. Voehringer, D. et al. (2004) J. Biol. Chem. 279:54117.
  8. Shiratori, I. et al. (2005) J. Immunol. 175:4441.
  9. Cherwinski, H.M. et al. (2005) J. Immunol. 174:1348.
  10. Fallarino, F. et al. (2004) J. Immunol. 173:3748.
  11. Wright, G.J. et al. (2001) Immunology 102:173.
  12. Hoek, R.M. et al. (2000) Science 290:1768.
  13. Hatherley, D. and A.N. Barclay (2004) Eur. J. Immunol. 34:1688.
  14. Gorczynski, R. et al. (2004) J. Immunol. 172:7744.
  15. Jenmalm, M.C. et al. (2006) J. Immunol. 176:191.
  16. Zhang, S. et al. (2004) J. Immunol. 173:6786.
  17. Vaine, C.A. et al. (2014) Adv Immunol.121:191.
  18. Hernangómez, M. et al. (2014) Curr Pharm Des. 20:4707.

Long Name

CD200 Receptor 1

Alternate Names

CD200 R1, MOX2R

Entrez Gene IDs

131450 (Human); 57781 (Mouse); 102139962 (Cynomolgus Monkey)

Gene Symbol

CD200R1

UniProt

Q8TD46

Additional CD200R1 Products

Product Documents for Recombinant Human CD200R1 His-tag Protein, CF

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Product Specific Notices for Recombinant Human CD200R1 His-tag Protein, CF

For research use only

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