Recombinant Human CD24 Fc Chimera Avi-tag Protein, CF

R&D Systems, part of Bio-Techne | Catalog # AVI5247

Biotinylated
R&D Systems, part of Bio-Techne
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AVI5247-050
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Key Product Details

Learn more about Avi-tag Biotinylated Proteins

Source

CHO

Accession #

P25063.3

Structure / Form

Disulfide-linked homodimer, biotinylated via Avi-tag

Conjugate

Biotin

Applications

Bioactivity

View all CD24 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human CD24 protein
Human CD24
(Ser27-Gly59)
Accession # P25063.3		 IEGRMD Human IgG1
(Pro100-Lys330)		 Avi-tag
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ser27

Predicted Molecular Mass

32 kDa

SDS-PAGE

43-55 kDa, under reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human Siglec-10 Fc Chimera (Catalog # 2130-SL) is coated at 5 μg/mL, the concentration of Biotinylated Recombinant Human CD24 Fc Chimera Avi-tag (Catalog # AVI5247) that produces 50% optimal binding response is found to be approximately 1.5-7.5 μg/mL.

Scientific Data Images for Recombinant Human CD24 Fc Chimera Avi-tag Protein, CF

Biotinylated Recombinant Human CD24 Fc Chimera Avi-tag Binding Activity.

When Recombinant Human Siglec-10 Fc Chimera (2130-SL) is coated at 5 μg/mL, the concentration of Biotinylated Recombinant Human CD24 Fc Chimera Avi-tag (Catalog # AVI5247) that produces 50% optimal binding response is found to be approximately 1.5-7.5 μg/mL.
2 μg/lane of Biotinylated Recombinant Human CD24 Fc Chimera Avi-tag Protein (Catalog # AVI5247) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 43-55 kDa and 80-100 kDa, respectively.

Formulation, Preparation and Storage

AVI5247
Formulation Supplied as a 0.2 μm filtered solution in PBS. 
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD24

CD24, also known as Heat-Stable Antigen and Nectadrin, is a heavily and variably glycosylated 30 kDa-60 kDa GPI-linked sialoprotein (1-4). Human CD24 is expressed on B lineage cells and granulocytes, on epithelial, neuronal, and muscle cells, and on a range of tumor cells (4-6). In mouse, CD24 is even more widely expressed, particularly on T cells, monocytes, and dendritic cells (2). CD24 expression is regulated during lineage development and with the activation of various cell types (4). Antibody crosslinking of CD24 enhances the induction of apoptosis in B and T lymphocytes which contributes to negative selection and the induction of immune tolerance (7-11). CD24 on antigen presenting cells cooperates with B7 molecules in the co-stimulation of T cells (12-14). CD24 associates in cis with Siglec-10 (or Siglec-G in mouse) and with the danger-associated molecules HMGB1, HSP70, or HSP90 which are released from necrotic or damaged cells (15). Formation of these ternary complexes fills a protective role: the resulting Siglec-10 signaling inhibits inflammatory responses that are otherwise induced by extracellular DAMPs (15, 16). Mature human CD24 shares 30% and 42% amino acid sequence identity with mouse and rat CD24, respectively. Our Avi-tag Biotinylated human CD24 features biotinylation at a single site contained within the Avi-tag, a unique 15 amino acid peptide. Protein orientation will be uniform when bound to streptavidin-coated surface due to the precise control of biotinylation and the rest of the protein is unchanged so there is no interference in the protein's bioactivity.

References

  1. Jackson, D. et al. (1992) Cancer Res. 52:5264.
  2. Kay, R. et al. (1991) J. Immunol. 147:1412.
  3. Kadmon, G. et al. (1992) J. Cell Biol. 118:1245.
  4. Fang, X. et al. (2010) Cell. Mol. Immunol. 7:100.
  5. Fischer, G. F. et al. (1990) J. Immunol. 144:638.
  6. Allman, D.M. et al. (1993) J. Immunol. 151:4431.
  7. Suzuki, T. et al. (2001) J. Immunol. 166:5567.
  8. Taguchi, T. et al. (2003) J. Immunol. 170:252.
  9. Jung, K.C. et al. (2004) J. Immunol. 172:795.
  10. Kishimoto, H. and J. Sprent (1997) J. Exp. Med. 185:263.
  11. Hitsumoto, Y. et al. (1996) Immunology 89:200.
  12. Liu, Y. et al. (1992) Eur. J. Immunol. 22:2855.
  13. Enk, A.H. and S.I. Katz (1994) J. Immunol. 152:3264.
  14. Askew, D. and C.V. Harding (2008) Immunology 123:447.
  15. Chen G.Y. et al. (2009) Science 323:1722.
  16. Liu, Y. et al. (2011) Curr. Opin. Immunol. 23:41.

Alternate Names

CD24, CD24A

Entrez Gene IDs

100133941 (Human); 12484 (Mouse)

Gene Symbol

CD24

UniProt

P25063.3

Additional CD24 Products

Product Documents for Recombinant Human CD24 Fc Chimera Avi-tag Protein, CF

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Human CD24 Fc Chimera Avi-tag Protein, CF

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