Recombinant Human CD27/TNFRSF7 Fc Avi-tag Protein, CF

R&D Systems, part of Bio-Techne | Catalog # AVI11027

Biotinylated
R&D Systems, part of Bio-Techne
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AVI11027-050
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Key Product Details

Learn more about Avi-tag Biotinylated Proteins

Source

CHO

Accession #

P26842.2

Structure / Form

Disulfide-linked homodimer
Biotinylated via Avi-tag

Conjugate

Biotin

Applications

Bioactivity

View all CD27/TNFRSF7 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human CD27/TNFRSF7 protein
Human CD27
(Thr21-Arg191)
Accession # P26842.2		 IEGRMD Human IgG1 Fc
(Pro100-Lys330)		 Avi-tag
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Thr21

Predicted Molecular Mass

48 kDa

SDS-PAGE

57-69 kDa, under reducing conditions.

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human CD27 Ligand/TNFSF7 Protein (Catalog # 9328-CL) is immobilized at 1.0 µg/mL (100 µL/well), Biotinylated Recombinant Human CD27/TNFRSF7 Fc Chimera Avi-tag (Catalog # AVI11027) binds with an ED50 of 6.00-60.0 ng/mL.

Scientific Data Images for Recombinant Human CD27/TNFRSF7 Fc Avi-tag Protein, CF

Biotinylated Recombinant Human CD27/TNFRSF7 Fc Chimera Avi-tag Protein SDS-PAGE.

2 μg/lane of Biotinylated Recombinant Human CD27/TNFRSF7 Fc Chimera Avi-tag Protein (Catalog # AVI11027) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 57-69 kDa and 110-140 kDa, respectively.

Formulation, Preparation and Storage

AVI11027
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD27/TNFRSF7

CD27, also known as TNFRSF7, is an approximately 55 kDa transmembrane protein in the TNF receptor superfamily. It functions as a co‑stimulatory molecule that supports lymphocyte activation and survival (1). Mature human CD27 consists of a 172 amino acid (aa) extracellular domain (ECD) with three TNFR cysteine‑rich repeats, a 21 aa transmembrane segment, and a 48 aa cytoplasmic domain (2). Within the ECD, human CD27 shares 63% and 66% aa sequence identity with mouse and rat CD27, respectively. CD27 is expressed as a disulfide‑linked homodimer that carries N‑linked and O‑linked glycosylation (3, 4). Proteolytic cleavage of CD27 results in the shedding of a 28‑32 kDa fragment of the ECD (4). CD27 is weakly expressed on naïve T cells and NK cells and is up‑regulated upon cell activation (4, 5). It is also up‑regulated on activated germinal center B cells, plasma cells, and a subset of memory B cells (6, 7). CD27 binds to the transmembrane glycoprotein CD27 Ligand/CD70 which is expressed on activated B cells, activated T cells, and dendritic cells (1, 8, 9). This interaction contributes to the activation and survival of CD4+ helper T cells (8‑11), CD8+ effector T cells (12, 13), memory T cells (10), and NK cells (5, 14). Ligation of CD27 on B cells promotes germinal center formation and the expansion and affinity maturation of memory B cell responses (6, 15). Our Avi-tag Biotinylated CD27/TNFRSF7 Fc Chimeria features biotinylation at a single site contained within the Avi-tag, a unique 15 amino acid peptide. Protein orientation will be uniform when bound to streptavidin-coated surface due to the precise control of biotinylation and the rest of the protein is unchanged so there is no interference in the protein's bioactivity.

References

  1. Nolte, M.A. et al. (2009) Immunol. Rev. 229:216.
  2. Camerini, D. et al. (1991) J. Immunol. 147:3165.
  3. van Lier, R.A. et al. (1987) J. Immunol. 139:1589.
  4. Loenen, W.A. et al. (1992) Eur. J. Immunol. 22:447.
  5. Takeda, K. et al. (2000) J. Immunol. 164:1741.
  6. Xiao, Y. et al. (2004) J. Immunol. 172:7432.
  7. Jung, J. et al. (2000) Eur. J. Immunol. 30:2437.
  8. Goodwin, R.G. et al. (1993) Cell 73:447.
  9. Bowman, M.R. et al. (1994) J. Immunol. 152:1756.
  10. Hendriks, J. et al. (2000) Nat. Immunol. 1:433.
  11. van Oosterwijk, M.F. et al. (2007) Int. Immunol. 19:713.
  12. Rowley, T.F. and A. Al Shamkhani (2004) J. Immunol. 172:6039.
  13. Hendriks, J. et al. (2003) J. Exp. Med. 198:1369.
  14. Kelly, J.M. et al. (2002) Nat. Immunol. 3:83.
  15. Raman, V.S. et al. (2003) J. Immunol. 171:5876.

Alternate Names

CD27, TNFRSF7

Entrez Gene IDs

939 (Human); 21940 (Mouse); 500318 (Rat); 102139476 (Cynomolgus Monkey)

Gene Symbol

CD27

UniProt

P26842.2

Additional CD27/TNFRSF7 Products

Product Documents for Recombinant Human CD27/TNFRSF7 Fc Avi-tag Protein, CF

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Human CD27/TNFRSF7 Fc Avi-tag Protein, CF

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