Recombinant Human CD27/TNFRSF7 Fc Chimera Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 382-CD

R&D Systems, part of Bio-Techne
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382-CD-100
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Key Product Details

Source

NS0

Accession #

P26842

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all CD27/TNFRSF7 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human CD27/TNFRSF7 protein
Human CD27
(Thr21-Ile192)
Accession # P26842		 EGRMD Human IgG1
(Pro100-Lys330)		 6-His tag
N-terminus C-terminus

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Thr21

Predicted Molecular Mass

46.5 kDa (monomer)

SDS-PAGE

60-70 kDa, reducing conditions

Activity

Measured by its ability to inhibit the proliferation of mouse T cells induced by CD27 Ligand and anti-CD3.
The ED50 for this effect is 0.75-3 µg/mL in the presence of 10 µg/mL (100 µL/well) Recombinant Mouse CD27 Ligand/TNFSF7 (Catalog # 783-CL) and anti-CD3.

Formulation, Preparation and Storage

382-CD
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 100 μg/mL in sterile PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD27/TNFRSF7

CD27, also known as TNFRSF7, is an approximately 55 kDa transmembrane protein in the TNF receptor superfamily. It functions as a co‑stimulatory molecule that supports lymphocyte activation and survival (1). Mature human CD27 consists of a 172 amino acid (aa) extracellular domain (ECD) with three TNFR cysteine‑rich repeats, a 21 aa transmembrane segment, and a 48 aa cytoplasmic domain (2). Within the ECD, human CD27 shares 63% and 66% aa sequence identity with mouse and rat CD27, respectively. CD27 is expressed as a disulfide‑linked homodimer that carries N‑linked and O‑linked glycosylation (3, 4). Proteolytic cleavage of CD27 results in the shedding of a 28‑32 kDa fragment of the ECD (4). CD27 is weakly expressed on naïve T cells and NK cells and is up‑regulated upon cell activation (4, 5). It is also up‑regulated on activated germinal center B cells, plasma cells, and a subset of memory B cells (6, 7). CD27 binds to the transmembrane glycoprotein CD27 Ligand/CD70 which is expressed on activated B cells, activated T cells, and dendritic cells (1, 8, 9). This interaction contributes to the activation and survival of CD4+ helper T cells (8‑11), CD8+ effector T cells (12, 13), memory T cells (10), and NK cells (5, 14). Ligation of CD27 on B cells promotes germinal center formation and the expansion and affinity maturation of memory B cell responses (6, 15).

References

  1. Nolte, M.A. et al. (2009) Immunol. Rev. 229:216.
  2. Camerini, D. et al. (1991) J. Immunol. 147:3165.
  3. van Lier, R.A. et al. (1987) J. Immunol. 139:1589.
  4. Loenen, W.A. et al. (1992) Eur. J. Immunol. 22:447.
  5. Takeda, K. et al. (2000) J. Immunol. 164:1741.
  6. Xiao, Y. et al. (2004) J. Immunol. 172:7432.
  7. Jung, J. et al. (2000) Eur. J. Immunol. 30:2437.
  8. Goodwin, R.G. et al. (1993) Cell 73:447.
  9. Bowman, M.R. et al. (1994) J. Immunol. 152:1756.
  10. Hendriks, J. et al. (2000) Nat. Immunol. 1:433.
  11. van Oosterwijk, M.F. et al. (2007) Int. Immunol. 19:713.
  12. Rowley, T.F. and A. Al Shamkhani (2004) J. Immunol. 172:6039.
  13. Hendriks, J. et al. (2003) J. Exp. Med. 198:1369.
  14. Kelly, J.M. et al. (2002) Nat. Immunol. 3:83.
  15. Raman, V.S. et al. (2003) J. Immunol. 171:5876.

Alternate Names

CD27, TNFRSF7

Entrez Gene IDs

939 (Human); 21940 (Mouse); 500318 (Rat); 102139476 (Cynomolgus Monkey)

Gene Symbol

CD27

UniProt

P26842

Additional CD27/TNFRSF7 Products

Product Documents for Recombinant Human CD27/TNFRSF7 Fc Chimera Protein, CF

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Human CD27/TNFRSF7 Fc Chimera Protein, CF

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