Recombinant Human CD277/BTN3A1 Fc Avi-tag Protein, CF

R&D Systems, part of Bio-Techne | Catalog # AVI8539

Fc Chimera
R&D Systems, part of Bio-Techne
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AVI8539-050
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Key Product Details

Source

CHO

Structure / Form

Disulfide-linked homodimer
Biotinylated via Avi-tag

Conjugate

Biotin

Applications

Bioactivity

View all CD277/BTN3A1 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human CD277/BTN3A1 protein
Human CD277/BTN3A1
(Gln30-Gly254)
Accession # O00481.3		 IEGRMD Human IgG1
(Pro100-Lys330)		 Avi-tag
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Gln30 inferred from enzymatic pyroglutamate treatment revealing Phe31

Predicted Molecular Mass

53 kDa

SDS-PAGE

57-65 kDa, under reducing conditions.

Activity

Measured by its binding ability in a functional ELISA.
Biotinylated Recombinant Human CD277/BTN3A1 Fc Chimera Avi-tag binds to Human BTN3A1/2/3 Antibody (Catalog # MAB7136) with an ED50 of 0.500-5.00 ng/mL.

Scientific Data Images for Recombinant Human CD277/BTN3A1 Fc Avi-tag Protein, CF

Biotinylated Recombinant Human CD277/BTN3A1 Fc Chimera Avi-tag Protein Binding Activity.

Measured by its binding ability in a functional ELISA. Biotinylated Recombinant Human CD277/BTN3A1 Fc Chimera Avi-tag Protein (Catalog # AVI8539) binds to Human BTN3A1/2/3 Antibody (MAB7136) with an ED50 of 0.500-5.00 ng/mL.

Biotinylated Recombinant Human CD277/BTN3A1 Fc Chimera Avi-tag Protein SDS-PAGE.

2 μg/lane of Biotinylated Recombinant Human CD277/BTN3A1 Fc Chimera Avi-tag Protein (Catalog # AVI8539) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 57-65 kDa and 110-130 kDa, respectively.

Formulation, Preparation and Storage

AVI8539
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD277/BTN3A1

Butyrophilin 3A1 (also called BTN3A1) is a 57 kDa type I transmembrane glycoprotein member of the Ig superfamily. It is expressed on a wide variety of immune cells. Similar to BTN3A2 and BTN3A3, BTN3A1 (484 amino acids) is composed of an extracellular N-terminal IgV and a membrane-proximal IgC domain followed by a transmembrane domain and a cytoplasmic tail. These Ig domains are also found in B7 family co-stimulatory molecules, suggesting structural and functional similarities between the two protein families (1). The intracellular portion of BTN3A1 contains a B30.2 domain (2). Although the B30.2 domain of BTN1A1 binds to xanthine oxidoreductase (XOR) and is conserved among BTN1A1 orthologs, this interaction with XOR is not shared by BTN3A1 (3). The B30.2 domain of butyrophilins also functions as a sensor for detecting changes in intracellular phopho-antigen (pAg) concentrations produced during tumorigenesis and microbial infections (4, 5). The specific binding of pAg by the B30.2 domain of BTN3A1 induces a conformational change in its ECD, leading to the activation of V gamma9V delta2 T cells (6). Thus, BTN3A1 acts as a critical protein for the activation of V gamma9V delta2 T cells following detection of distressed cells (7). The anti-tumor responses of V gamma9V delta2 T cells may be enhanced with agonistic anti-BTNA3 antibodies (8). No BTN3A1 homolog has yet been identified in rodents. However, Human BTN3A1 shares 92.4% and 91.6% sequence identity with baboon and rhesus monkey BTN3A1 respectively (9).  Our Avi-tag Biotinylated  human BTN3A1 Fc chimera features biotinylation at a single site contained within the Avi-tag, a unique 15 amino acid peptide. Protein orientation will be uniform when bound to streptavidin-coated surface due to the precise control of biotinylation and the rest of the protein is unchanged so there is no interference in the protein's bioactivity.

References

  1. Abeler-Dorner, L. et al. (2012) Trends Immunol. 33:34.
  2. Rhodes, D.A. et al. (2001) Genomics 71:351.
  3. Jeong, J. et al. (2009) J. Biol. Chem. 284:22444.
  4. Harly, C. et al. (2012) Blood 120:2269.
  5. Constant, P. et al. (1994) Science 264:267.
  6. Sandstrom, A. et al. (2014) Immunity 40:490.
  7. Harly, C. et al. (2015) Front. Immunol. 5:657.
  8. Bonneville, M. et al. (2006) Curr. Opin. Immunol. 18:539.
  9. Wang, H. et al. (2013) J.Immunol. 191:1029.

Alternate Names

BT3.1, BTF5, BTN3A1, CD277

Entrez Gene IDs

11119 (Human)

Gene Symbol

BTN3A1

Additional CD277/BTN3A1 Products

Product Documents for Recombinant Human CD277/BTN3A1 Fc Avi-tag Protein, CF

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Human CD277/BTN3A1 Fc Avi-tag Protein, CF

For research use only

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