Recombinant Human CD37 Fc Chimera Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 10160-CD

R&D Systems, part of Bio-Techne
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10160-CD-050
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Key Product Details

Source

CHO

Accession #

P11049

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all CD37 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human CD37 protein
Human CD37
(Thr110-Asn241)
Accession # P11049
 IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Thr110

Predicted Molecular Mass

42 kDa

SDS-PAGE

54-63 kDa, under reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human CD37 Fc Chimera (Catalog # 10160-CD) is immobilized at 10 μg/mL (100 μL/well), the concentration of Biotinylated Recombinant Human CD19 Fc Chimera Avi-tag that produces 50% of the optimal binding response is 5-25 μg/mL.

Scientific Data Images for Recombinant Human CD37 Fc Chimera Protein, CF

Recombinant Human CD37 Fc Chimera Protein SDS-PAGE

Recombinant Human CD37 Fc Chimera Protein SDS-PAGE

2 μg/lane of Recombinant Human CD37 Fc Chimera (Catalog # 10160-CD) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing major bands at 54-63 kDa and 108-126 kDa, respectively.

Formulation, Preparation and Storage

10160-CD
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD37

CD37, also known as TSPAN26, is a 40‑45 kDa palmitoylated traspanin superfamily glycoprotein that is mainly expressed by B cell lymphocytes and myeloid cells (1). Tetraspanins are characterized by four transmembrane domains, one small extracellular loop (SEL) and one large extracellular loop (LEL), and short N- and C-termini (1). The CD37-LEL has the most variation across these different domains, but a CCG motif and the position of the last cysteine residue in the LEL are conserved in all tetraspanins (2). Tetraspanins form complexes with a wide range of transmembrane and cytoplasmic proteins, including integrins, forming small networks referred to as tetraspanin enriched microdomains, or TEMs (1). Human CD37-LEL shares 66% amino acid sequence identity with mouse and rat CD37. CD37 directly associates with dectin-1 on macrophages and stabilizes cell surface dectin-1 expression (3). It also inhibits T cell activation and proliferation by limiting T cell receptor signaling (4). Mice deficient in CD37 develop B cell lymphoma (5). Kidneys of CD37-/- mice showed more mesangial proliferation, endothelial cell activation, podocyte activation, and segmental podocyte foot process effacement compared to the CD37/Il6 double-knockout mice, emphasizing that IL-6 mediates renal pathology in CD37-/- mice. Thus, CD37 may protect against IgA nephropathy by inhibition of the IL-6 pathway (6).

References

  1. Charrin, S. et al. (2002) FEBS Lett. 516:139.
  2. Berditchevski, Fedor. (2001) J. Cell. Sci. 114:4143.
  3. Meyer-Wentrup, F. et al. (2007) J Immunol. 178:154.
  4. van Spriel, A.B. et al. (2004) J Immunol 172:2953.
  5. de Winde, C.M. et al. (2016) J Clin Invest. 126:653.
  6. Rops, ALWMM et al. (2018) Kidney Int. 93:1356.

Alternate Names

CD37, TSPAN26

Entrez Gene IDs

951 (Human); 12493 (Mouse); 29185 (Rat)

Gene Symbol

CD37

UniProt

P11049

Additional CD37 Products

Product Documents for Recombinant Human CD37 Fc Chimera Protein, CF

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Human CD37 Fc Chimera Protein, CF

For research use only

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