Recombinant Human CD38 Fc Chimera Avi-tag Protein, CF
R&D Systems, part of Bio-Techne | Catalog # AVI10920
Key Product Details
Learn more about Avi-tag Biotinylated Proteins
Source
HEK293
Accession #
Structure / Form
Biotinylated via Avi-tag.
Conjugate
Biotin
Applications
Bioactivity, Enzyme Activity
Product Specifications
Source
Human embryonic kidney cell, HEK293-derived human CD38 protein
Human CD38 (Val43-Ile300) Accession # P28907.2 |
IEGRMD | Human IgG1 (Pro100-Lys330) |
Avi-tag |
N-terminus | C-terminus |
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Val43
Predicted Molecular Mass
58 kDa
SDS-PAGE
69-77 kDa, under reducing conditions.
Activity
Measured by its binding ability in a functional ELISA.
When Human CD38 Antibody (Catalog # AF2404) is immobilized at 1.00 μg/mL (100 µL/well), Biotinylated Recombinant Human CD38 Fc Chimera Avi-tag binds with an ED50 of 1.00-6.00 ng/mL.
Measured by its ability to convert the substrate nicotinamide guanine dinucleotide (NGD+) to cyclic GDP-ribose. Graeff, R.M. et al. (1994) J. Biol. Chem. 269:30260.
The specific activity is >700 pmol/min/μg, as measured under the described conditions.
When Human CD38 Antibody (Catalog # AF2404) is immobilized at 1.00 μg/mL (100 µL/well), Biotinylated Recombinant Human CD38 Fc Chimera Avi-tag binds with an ED50 of 1.00-6.00 ng/mL.
Measured by its ability to convert the substrate nicotinamide guanine dinucleotide (NGD+) to cyclic GDP-ribose. Graeff, R.M. et al. (1994) J. Biol. Chem. 269:30260.
The specific activity is >700 pmol/min/μg, as measured under the described conditions.
Scientific Data Images for Recombinant Human CD38 Fc Chimera Avi-tag Protein, CF
Biotinylated Recombinant Human CD38 Fc Chimera Avi-tag Protein SDS-PAGE.
2 μg/lane of Biotinylated Recombinant Human CD38 Fc Chimera Avi-tag Protein (Catalog # AVI10920) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 69-77 kDa and 140-150 kDa, respectively.Formulation, Preparation and Storage
AVI10920
Formulation | Supplied as a 0.2 μm filtered solution in MES and NaCl. |
Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: CD38
References
- Schuber, F. and F.E. Lund (2004) Curr. Mol. Med. 4:249.
- Liu, Q. et al. (2005) Structure. 13:1331.
- Jackson, D.G. and J.I. Bell (1990) J. Immunol. 144:2811.
- Sun, L. et al. (1999) J. Cell Biol. 146:1161.
- Partida-Sanchez, S. et al. (2001) Nature Med. 7:1209.
- Kato, I. et al. (1995) J. Biol. Chem. 270:30045.
- Deaglio, S. (2000) Chem. Immunol. 75:99.
- Chillemi,A. et al. (2013) Mol. Med. 19:99.
- Vaisitti, T. et al. (2015) Leukemia. 29:356.
- Atanackovic, D. et al. (2016) Oncoimmunology. 5:e1217374.
- Tai, Y-T. et al. (2017) Oncotarget. 8:112166.
Long Name
Cluster of Differentiation 38
Alternate Names
ADP-ribosyl Cyclase, CD38, Cyclic ADP-ribose Hydrolase
Gene Symbol
CD38
UniProt
Additional CD38 Products
Product Documents for Recombinant Human CD38 Fc Chimera Avi-tag Protein, CF
Product Specific Notices for Recombinant Human CD38 Fc Chimera Avi-tag Protein, CF
For research use only
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