Recombinant Human CD39L1/ENTPD2 Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 6087-EN
Key Product Details
Source
CHO
Accession #
Structure / Form
Monomer
Conjugate
Unconjugated
Applications
Enzyme Activity
Product Specifications
Source
Chinese Hamster Ovary cell line, CHO-derived human CD39L1/ENTPD2 protein
Thr29-Asp460, with a C-terminal 6-His tag
Thr29-Asp460, with a C-terminal 6-His tag
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Thr29
Predicted Molecular Mass
48 kDa
SDS-PAGE
60-70 kDa, reducing conditions
Activity
Measured by its ability to hydrolyze the 5’-phosphate groups from the substrate adenosine-5’-triphosphate (ATP). The orthophosphate product is measured by a Malachite Green Phosphate Detection Kit (Catalog # DY996).
The specific activity is >5,000 pmol/min/μg, as measured under the described conditions.
The specific activity is >5,000 pmol/min/μg, as measured under the described conditions.
Formulation, Preparation and Storage
6087-EN
| Formulation | Supplied as a 0.2 μm filtered solution in Tris, NaCl, CaCl2 and Glycerol. |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: CD39L1/ENTPD2
CD39L1, also known as ENTPD2 and NTPDase2, is an ectonucleotidase belonging to the CD39 family. It is found on the surface of vascular adventitial cells and accessory vascular cells (1). CD39L1 is a Ca2+ and Mg2+ dependent enzyme that activates platelets by preferentially converting ATP to ADP (2). CD39L1 plays a role in regulating thrombosis and inflammation (3). It is considered to be a therapeutic target for thromboregulation and the treatment of vascular inflammation (2, 4).
References
Zimmermann, H. et al. 2000 Proceedings of the Second International Workshop on EctoATPases and Related Ectonucleotidases:18.
Robson, S.C. et al. 2001 Drug Dev. Res. 53:193.
Marcus, A.J. et al. 2005 Semin. Thromb. Hemost. 31:234.
Sevigny, J. et al. 2002 Blood 99:2801.
Long Name
Ecto-Nucleoside Triphosphate Diphosphohydrolase 2
Alternate Names
CD39L1, Ecto-ATPase, ENTPD2, NTPD2, NTPDase 2
Gene Symbol
ENTPD2
UniProt
Additional CD39L1/ENTPD2 Products
Product Documents for Recombinant Human CD39L1/ENTPD2 Protein, CF
Product Specific Notices for Recombinant Human CD39L1/ENTPD2 Protein, CF
For research use only
Loading...
Loading...
Loading...