Recombinant Human CD39L4/ENTPD5 Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 5297-EN
Key Product Details
Product Specifications
Source
Chinese Hamster Ovary cell line, CHO-derived human CD39L4/ENTPD5 protein
Val21-His428 (major) & Ser46-His428 (minor), both with a C-terminal 6-His tag
Accession # O75356
Val21-His428 (major) & Ser46-His428 (minor), both with a C-terminal 6-His tag
Accession # O75356
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Val21 & Ser46
Predicted Molecular Mass
46 kDa
SDS-PAGE
50-60 kDa, reducing conditions
Activity
Measured by its ability to hydrolyze the 5’-phosphate groups from the substrate guanosine-5’-diphosphate (GDP). The orthophosphate product is measured by a Malachite Green Phosphate Detection Kit (Catalog # DY996).
The specific activity is >7,000 pmol/min/µg, as measured under the described conditions.
The specific activity is >7,000 pmol/min/µg, as measured under the described conditions.
Formulation, Preparation and Storage
5297-EN
| Formulation | Supplied as a 0.2 μm filtered solution in Tris, CaCl2, NaCl and Glycerol. |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: CD39L4/ENTPD5
Ectonucleoside triphosphate diphosphohydrolase-5 (NTPDase-5) is a protein that can be either secreted or reside in the lumen of the endoplasmic reticulum. The enzyme displays a specificity for nucleoside-5'-diphosphates, preferentially hydrolyzing GDP, IDP, and UDP to produce nucleoside monophosphates and orthophosphate (1). The enzyme is most highly expressed in liver, kidney, prostate, testis, and colon. NTPDase-5 (ENTPD5) and proto-oncogene CPH (PCPH) are encoded by the same gene (2). Alterations in ENTPD5/PCPH expression and mutations of ENTPD5 are associated with the progression of testicular (3), laryngeal (4), and other cancers.
References
- Mulero, J.J. et al. (2000) Biochemistry. 39:12924.
- Paez, J.G.. et al. (2001) Int. J. Oncol. 19:1249.
- Regadera, J. et al. (2006) Int. J. Oncol. 28:595.
- Blanquez, M.J. et al. (2002) Mol. Carcinogen. 35:186.
Long Name
Ecto-Nucleoside Triphosphate Diphosphohydrolase 5
Alternate Names
CD39L4, ENTPD5, ER-UDPase, NTPDase 5, PCPH
Gene Symbol
ENTPD5
UniProt
Additional CD39L4/ENTPD5 Products
Product Documents for Recombinant Human CD39L4/ENTPD5 Protein, CF
Product Specific Notices for Recombinant Human CD39L4/ENTPD5 Protein, CF
For research use only
Loading...
Loading...
Loading...