Recombinant Human CD4 Fc Chimera Avi-tag Protein, CF
R&D Systems, part of Bio-Techne | Catalog # AVI10965
Biotinylated
Key Product Details
Learn more about Avi-tag Biotinylated Proteins
Source
CHO
Accession #
Structure / Form
Disulfide-linked homodimer
Biotinylated via Avi-tag
Biotinylated via Avi-tag
Conjugate
Biotin
Applications
Bioactivity
Product Specifications
Source
Chinese Hamster Ovary cell line, CHO-derived human CD4 protein
Human CD4 (Lys26-Trp390) Accession # P01730.1 |
IEGRMD | Human IgG1 Fc (Pro100-Lys330) |
Avi-tag |
N-terminus | C-terminus |
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Lys26
Predicted Molecular Mass
69 kDa
SDS-PAGE
78-87 kDa, under reducing conditions.
Activity
Measured by its binding ability in a functional ELISA.
When Human CD4 Antibody (Catalog # AF-379-NA) is immobilized at 2.00 μg/mL (100 μL/well), Biotinylated Recombinant Human CD4 Fc Chimera Avi-tag binds with an ED50 of 2.00-12.0 ng/mL.
When Human CD4 Antibody (Catalog # AF-379-NA) is immobilized at 2.00 μg/mL (100 μL/well), Biotinylated Recombinant Human CD4 Fc Chimera Avi-tag binds with an ED50 of 2.00-12.0 ng/mL.
Scientific Data Images for Recombinant Human CD4 Fc Chimera Avi-tag Protein, CF
Biotinylated Recombinant Human CD4 Fc Chimera Avi-tag Protein Binding Activity.
When Human CD4 Antibody (AF-379-NA) is immobilized at 2.00 μg/mL (100 μL/well), Biotinylated Recombinant Human CD4 Fc Chimera Avi-tag Protein (Catalog # AVI10965) binds with an ED50 of 2.00-12.0 ng/mL.Biotinylated Recombinant Human CD4 Fc Chimera Avi-tag Protein SDS-PAGE.
2 μg/lane of Biotinylated Recombinant Human CD4 Fc Chimera Avi-tag Protein (Catalog # AVI10965) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 78-87 kDa and 160-170 kDa, respectively.Formulation, Preparation and Storage
AVI10965
Formulation | Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. |
Reconstitution | Reconstitute at 500 μg/mL in PBS. |
Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: CD4
References
- Vignali, D.A.A. (2010) J. Immunol. 184:5933.
- Maddon, P.J. et al. (1985) Cell 42:93.
- Alarcon, B. and H.M. van Santen (2010) Sci. Signal. 3:pe11.
- Wan, Y.Y. and R.A. Flavell (2009) Mol. Cell Biol. 1:20.
- Kitchen, S.G. et al. (2005) Proc. Natl. Acad. Sci. 102:3794.
- Crocker, P.R. et al. (1987) J. Exp. Med. 166:613.
- Biswas, P. et al. (2003) Blood 101:4452.
- Bernstein, H.B. et al. (2006) J. Immunol. 177:3669.
- Funke, I. et al. (1987) J. Exp. Med. 165:1230.
- Doyle, C. and J.L. Strominger (1987) Nature 330:256.
- Huppa, J.B. et al. (2010) Nature 463:963.
- Fragoso, R. et al. (2003) J. Immunol. 170:913.
- Cruikshank, W.W. et al. (1994) Proc. Natl. Acad. Sci. 91:5109.
- Klatzmann, D. et al. (1984) Nature 312:767.
- Dagleish, A.G. et al. (1984) Nature 312:763.
Alternate Names
CD4
Entrez Gene IDs
Gene Symbol
CD4
UniProt
Additional CD4 Products
Product Documents for Recombinant Human CD4 Fc Chimera Avi-tag Protein, CF
Product Specific Notices for Recombinant Human CD4 Fc Chimera Avi-tag Protein, CF
For research use only
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