Recombinant Human CD40/TNFRSF5 Fc Avi-tag Protein, CF

R&D Systems, part of Bio-Techne | Catalog # AVI10380

R&D Systems, part of Bio-Techne
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AVI10380-050
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Key Product Details

Learn more about Avi-tag Biotinylated Proteins

Source

CHO

Accession #

P25942.1

Structure / Form

Disulfide-linked homodimer, biotinylated via Avi-tag

Conjugate

Biotin

Applications

Bioactivity

View all CD40/TNFRSF5 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human CD40/TNFRSF5 protein
Human CD40/TNFRSF5
(Glu21-Arg193)
Accession # P25942.1		 IEGRMD Human IgG1
(Pro100-Lys330)		 Avi-tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Glu21

Predicted Molecular Mass

48 kDa

SDS-PAGE

61-66 kDa, under reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human CD40 Ligand/TNFSF5 Fc (HEK293-expressed, (Catalog # 6420-CL) mobilized at 2 μg/mL (100 μL/well), the concentration of Recombinant Human CD40/TNFRSF5 Fc Chimera Avi-tag (Catalog # AVI10380) that produces 50% optimal binding is 0.0400-0.320 μg/mL.

Scientific Data Images for Recombinant Human CD40/TNFRSF5 Fc Avi-tag Protein, CF

Recombinant Human CD40/TNFRSF5 Fc Avi-tag Protein Binding Activity

Recombinant Human CD40/TNFRSF5 Fc Avi-tag Protein Binding Activity

When Recombinant Human CD40 Ligand/TNFSF5 (HEK293-expressed) Protein (Catalog # 6420-CL) is immobilized at 2 µg/mL, 100 µL/well, the concentration of Biotinylated Recombinant Human CD40/TNFRSF5 Fc Chimera Avi-tag (Catalog # AVI10380) that produces 50% optimal binding is 0.0400-0.320 μg/mL.
Recombinant Human CD40/TNFRSF5 Fc Avi-tag Protein SDS-PAGE

Recombinant Human CD40/TNFRSF5 Fc Avi-tag Protein SDS-PAGE

2 μg/lane of Biotinylated Recombinant Human CD40/TNFRSF5 Fc Chimera Avi-tag (Catalog # AVI10380) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 61-66 kDa and 120-135 kDa, respectively.

Formulation, Preparation and Storage

AVI10380
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD40/TNFRSF5

CD40, also known as TNFRSF5, is a 45-50 kDa type I transmembrane glycoprotein member of the TNF receptor superfamily (1). Mature human CD40 consists of a 173 amino acid (aa) extracellular domain, a transmembrane domain, and a 62 aa cytoplasmic domain (2). The extracellular domain of human CD40 shares 58% and 56% aa sequence identity with mouse and rat CD40, respectively. An antagonistic soluble human CD40 splice variant contains an alternate sequence within the extracellular and transmembrane domains and lacks a cytoplasmic domain (3). CD40 is expressed on the surface of B cells, dendritic cells, macrophages, monocytes and platelets, as well as endothelial and epithelial cells (4, 5). Interaction of CD40 with its ligand, CD40 Ligand, leads to the aggregation of CD40 molecules resulting in the initiation of bidirectional intracellular signaling in both CD40 and CD40 Ligand expressing cells (6). CD40 ligation by CD40 Ligand promotes B cell activation and T cell-dependent humoral responses (7, 8). CD40 serves multiple functions in both hematopoietic and epithelial cancers and is a target for tumor immunotherapy (9, 10). Dysregulation of CD40/CD40 Ligand expression and interactions contributes to the immune deficiency associated with HIV infection and AIDS (11, 12). It is also implicated in the pathology of multiple cardiovascular diseases including atherosclerosis, atherothrombosis, and restenosis (13, 14).

References

  1. Banchereau, J. et al. (1994) Annu. Rev. Immunol. 12:881.
  2. Stamenkovic, I. et al. (1989) EMBO J. 8:1403.
  3. Eshel, D. et al. (2008) Mol. Immunol. 46:250.
  4. van Kooten, C. and J. Banchereau (1997) Curr. Opin. Immunol. 9:330.
  5. Schonbeck, U. et al. (1997) J. Biol. Chem. 272:19569.
  6. Eissner, G. et al. (2004) Cytokine Growth Factor Rev. 15:353.
  7. Rickert, R.C. et al. (2011) Immunol. Rev. 244:115.
  8. Elgueta, R. et al. (2009) Immunol. Rev. 229:152.
  9. Loskog, A.S. and A.G. Eliopoulos (2009) Semin. Immunol. 21:301.
  10. Hangalapura, B.N. et al. (2012) J. Gene Med. 14:416.
  11. Kornbluth, R.S. (2000) J. Leukoc. Biol. 68:373.
  12. Chougnet, C. (2003) J. Leukoc. Biol. 74:702.
  13. Pamukcu, B. et al. (2011) Ann. Med. 43:331.
  14. Hassan, G.S. et al. (2012) Immunobiology 217:521.

Alternate Names

CD40, TNFRSF5

Entrez Gene IDs

958 (Human); 21939 (Mouse); 171369 (Rat); 102118696 (Cynomolgus Monkey)

Gene Symbol

CD40

UniProt

P25942.1

Additional CD40/TNFRSF5 Products

Product Documents for Recombinant Human CD40/TNFRSF5 Fc Avi-tag Protein, CF

Product Datasheets

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Product Specific Notices for Recombinant Human CD40/TNFRSF5 Fc Avi-tag Protein, CF

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